| Purpose and meaning: Caffeic acid phenethyl este(rCAPE), as one of the main active ingredients of the natural product propolis, shows the unique performance in physiological and pharmacological aspects, such as anti-tumor, anti-oxidation, anti-inflammatory, immune regulation, et al. Therefore, the research of CAPE, the active ingredient of propolis, turns to be a hot spark both at home and abroad in recent years. This paper is intended to be a reference for the new drug R&D, which take CAPE as the major effective component.Content: Through the research of a large number of domestic and foreign literatures, give pharmacology and pharmacodynamics of propolis and caffeic acid phenethyl ester in propolis a preliminary exploration. The propolis samples'rank is determined by the determination of propolis blocks'ethyl alcohol extraction content, the impurity and the beeswax content, the iodine value, the oxidized time, the phenol compound content and the flavonoid qualitative response. Propolis samples in this experiment are from the Hebei Pro. , China., which is determined by CAPE primitive consistency. This essay has suggested the convenient process routes of extracting CAPE from propolis. The materials and the methods for extracting CAPE of medium density from propolis are described, including the extractive solvent, the reaction temperature, the time of ultrasonic wave-extraction, the ratio between solid and liquid, the column chromatographic analysis response parameters and the correlation testing parameters. A RP-HPLC method for the determination of CAPE consistency has established. Through the antimicrobial tests of CAPE, its antibacterial laps and minimum inhibitory concentration come out. LD50 of mice has been got through CAPE samples'toxicological research. Make capsules with extract, and capsules were tested.Conclusion: The craft designed in this experiment can emolliently extract CAPE from bee propolis.Average density of CAPE samples can reach 48.75%, which is nearly 20 times comparing with original content of CAPE in the bee propolis samples (26.352mg/g, 2.64%), the returns-ratio is 2.38% and the extraction efficiency is 44.2%. Be showed from this, the craft route designed in this experiment completely can go towards withdrawing CAPE of medium density from the bee propolis. RP-HPLC method set up in this experiment can accurately measures samples'CAPE consistency. The CAPE extraction has the strong bacteriostasis activeness and the low toxicity. The capsules made of CAPE sample withdrawn in the bee propolis meets China medical dictionary provision. |
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