Changes Of TNF-a,IL-1βand IL-6 In Serum And Gingival Crevicular Fluid In Patients With Coronary Heart Disease Complicated With Periodontitis Before And After Periodontal Non-surgical Therapy

ObjectiveThe study analysed positive role of periodontal treatment to coronary heart disease through changes of TNF-a,IL-1βand IL-6 in serum and gingival crevicular fluid and clinical indicators before and after periodontal non-surgical therapy for periodontitis accompanied with coronary heart disease.It provides the basis in theory and experimentation for studying relativity and affecting factors between periodontitis and coronary heart disease.The necessity and effect of periodontal treatment for chronic periodontitis accompanied with coronary heart disease patients were also discussed.Materials and Methods1.Subjected of study:50 patients with coronary heart disease(stable stage) complicated with chronic periodontitis(CHD group),the age between 38-76.As the same time,50 patients with chronic periodontitis(CP group) and 50 people who were healthy in systematicly and orally(H group) were choosed.2.The basicline examination was carried out for three groups.It included periodontal probing depth(PD),clinical attachment loss(CAL),sulcus bleeding index(SBI) of existing full-mouth teeth and gathering the sample of serum and gingival crevicular fluid.3.Ultrasonic supragingival scaling was carried out for CHD group and CP group. Subgingival scaling and root planing were carried out for 2-4 times.It was necessary that the doctor communicated with patient and oral hygiene instruction for the patient. Antibiotics didn't recept for all patients.The baseline clinical periodontal examination was carried out after one month and three months when CHD group was treated,and CP group was treated after one month.The samples of serum and gingival crevicular fluid were acquired.The content of TNF-α和IL-1β,IL-6 were examined by the methodofELISA.3.Statistics analyse:the data was expressed by the mean value and standard deviation,and they were analysed by SPSS11.0 software.Results1.The baseline clinical indicators of periodontal PD,CAL,SBI in the CHD group and CP group was significantly higher than the H group(P＜0.01),while the CAL,SBI of the CHD group are higher than the CP group,the difference has statistical significance(P＜0.05)2.Three group's baseline serum tumor necrosis factor-a(TNF-a) and interleukin-1β(IL-1β)and interleukin-6(IL-6) levels were significantly different,CHD group and CP group were higher than and H group,and the CHD group was higher than CP group,the differences had statistical significance(P＜0.01);gingival crevicular fluid in the CHD group and CP group was significantly higher than in the H group,the differences were statistically significant(P＜0.01),but the CHD group and the CP group showed no significant difference(P＞0.05).3.CHD group and CP group showed a significant clinical improvement after periodontal non-surgical therapy.TNF-a and IL-1β,IL-6 levels of serum and gingival crevicular fluid had dropped,before and after treatment difference,it showed statistical significance(P＜0.01).ConclusionsThe level of inflammatory factors in serum were significantly related with that of the gingival crevicular fluid in both the coronary heart disease with chronic periodontitis and pure chronic periodontitis patients.The clinical effect after the periodontal therapy indicated that it could alleviate the whole inflammatory status of the patient with coronary heart disease complicated with chronic periodontitis,as well as improve cardiovascular function status.Periodontal therapy is essential for coronary heart disease with chronic periodontitis patients.Such a conclusion is benificial for the comprehensive treatment for coronary heart disease patients.