| Senile depression is a disease department to point a hair to expect in the old age of the obstacle of Senile depression, with showing the long moods low fall for a kind of function disease of the main clinical characteristic, belong to TCM "Senile depression " category. With today's society of the increasingly keen competition, the increasing pace of work to accelerate, the number of patients with Senile depression was significantly increased trend. Senile depression serious impact on people's life, work and health. At this stage for the treatment of Senile depression in Western medicine more categories, but the antidepressant effects are limited, easy to produce resistance and apparent side effects, making Western antidepressant was extremely restricted. Chinese medicine in the treatment of Senile depression in the process, gained a wealth of theoretical knowledge and clinical experience. In recent years, domestic Senile depression Chinese pathogenesis, diagnosis, prescription rule is, based on experiments conducted a more extensive and fruitful research, therefore, the theory of Chinese medicine, under the guidance of use modern scientific methods study medicine antidepressant effect and mechanism, find and secure development of antidepressant drugs is of great significance.Objective"Liver depression and phlegm" in the context of the Senile depression formed pathogenesis, Observation Chaiqin Wendan Decoction the unpredictability of chronic mild stress model of depression (CUMS) mice, reveal the right side of Senile depression effect and mechanism. MethodsAn experimental method of grouping : health Kunming 48 mice were divided into five groups : control group, model group, the positive drug control group (the control group), Chaiqin Wendan Decoction group, each with 12. Apart from the gaps in the remaining group were single cage feeding, according to the literature to establish chronic mild stress the unpredictability of Senile depression model. Drug Delivery Methods : Model 18 days, Chaiqin Wendan Decoction 1 group of mice were given the side decoction by rly (kg·d), 15g/ (kg·d) respectively, 0.1ml/10g; control group to amitriptyline 10mg (kg·d) volume suspension gavage; blank,model transcribed equal volume saline was administered by gavage for 18 days. BL-410 biological laboratory system to record the activities of the mice, 5min mice independent record number of activities and record the mice within 5 min stimulation frequency. Serotonin content HPLC detection technology, plasma cortisol concentrations were determined by radioimmunoassay.Results1 High Chai group, Chai low group and control group of mice autonomic activity increased significantly, Compared with the control group were significantly different (P <0.01); Chai high autonomy mice with a blank activities were not significantly different (P> 0.05); Low Chai group and the control group of mice compared to High Chai group was significantly higher (P <0.01); Low Chai group compared with the control group of mice autonomic activities no significant difference (P> 0.05).2 High Chai group, Chai low group and control group of mice significantly reduced the number of irritation , Compared with the control group were significantly different (P <0.01); Chai group of low irritable number compared with the control group were significantly different (P <0.01), Chai high group and the control group and the control group were not significantly different (P> 0.05); Chai low the number of irritation compared Chai high group and the control group were significantly different (P <0.01); Control group with high Chai group was no significant difference (P> 0.05)3 The Model group group of 5-HT was significantly reduced compared with the blank significant differences (P <0.01); High Chai group, Low Chai group and the control group of 5-HT was significantly higher compared with the model group were significantly different (P <0.01); High Chai group, Chai low group and control group of 5-HT content compared with blank were not significantly different (P> 0.05); control group compared with low group and high group had no significant difference (P> 0.05); Low Chai group of 5-HT was significantly lower than the high Chai group, compared with High Chai group significant difference (P <0.05).4 High Chai group, Chai low group and the control group of mice plasma cortisol concentration decreased significantly, Compared with the control group were significantly different (P <0.01); High Chai group, the control group of mice plasma cortisol concentrations compared with the control group had no significant difference (P> 0.05); Low Chai group significantly lower than control group of mice plasma cortisol concentration, compared with the blank exist significant differences (P <0.05); Chai Chai group and the high group had significantly lower (P <0.01) ,Compared with the control group are significantly different (P <0.05); Control group with low Chai group was not significantly different (P> 0.05).Conclusion1 Liver depression and phlegm are Senile depression important disease2 Chaiqin Wendan Decoction effective in improving Senile depression mice autonomy reduced, irritation and other vulnerable performance.3 Increased Senile depression mice brain tissues of 5-HT content, lower plasma cortisol concentrations are Chaiqin Wendan Decoction role of the Mechanism. |
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