Experimental Research On The Effect Of Anti-fatigue, Cold And Heat Resistance And Hypoxic Tolerance Of Hovenia Dulcis Thunb Water Extract And Preparations Of Its Reagent

Fatigue is a complex physiological phenomenon,which result in the reduction of the combat effectiveness of soldiers,and can lead to inefficiencies and errors increasing in accidents.If the fatigue can't recover in time,"Overtraining syndrome,OTS" and "Chronic fatigue syndrome,CFS" may occur.It leads to endocrine disturbance and immunity decreasing and threaten people's healthy.To enhance exercise endurance,and promote the recovery of fatigue,has become the new direction of the research on fatigue.Hypoxia refers to the activities of life that cannot get adequate oxygen,and hypoxia can induce the change of system and threaten human health.Cold,high temperature will affect the in vivo metabolism, too.They were also the elements affecting the combat effectiveness of troops.Therefore the drugs which will make people anti-fatigue,anti-cold,anti-heat and anti-hypoxia are the emphaese of medical research.To determine the total flavonoids and quercetin contents from the Semen Hoveniae extract(SHE),to investigate the Protective effect on oxidative damage and anti-fatigue effect of the extract,can provide theoretical evidence for further development of Semen Hoveniae.Hoveniae was extracted with water;KM mice were randomly divided into five groups: control group,positive group(Rhodiala),the low,the middle and the high dosage groups(50mg/kg,100mg/kg,200mg/kg).Few days later,high-temperature stimulation, cold stimulation,atmospheric hypoxia,sodium nitrite moderate hypoxia,acute cerebral ischemia and hypoxia and decompression hypoxia,pole climb,climb net,swimming and other model will be used to test the capacity of cold-resistant,heat-resistant,anti-anoxia and anti-fatigue of animals.To make corresponding Biochemical indicators-tests to test the glycogen storing up and the biochemistry target change which weary produces resulting from the movement.A PC 12 cell injury model induced by treatment with H₂O₂ for 18 hours was established to study the protective effect of Hoveniae.The content of total flavonoids was determined by chromatometry with Rutin as referenced substance.The content of quercetin in Hoveniae was quantitatively determined by High Performance Liquid Chromagraphy (HPLC).The experiment of cold-resisting and heat-resisting show that Hovenia dulcis Thunb Water Extract is efficient on cold-resisting and heat-resisting.The high-dose group improves significantly.The experiment of anti-hypoxia proves Hovenia dulcis Thunb Water Extract is able to extend survival time of mice under the condition of normal hypoxic,sodium nitrite moderate hypoxia,acute cerebral hypoxic-ischemic.And it can resist the biochemistry target change which weary produces because of hypoxia.The experiment of anti-fatigueprove that Hovenia dulcis Thunb Water Extract can strengthen the sports endurance,increases the and energy storage,resist the biochemistry target change such as Hb,LDH,SOD,GSH-px, T-AOC and MDA,which weary produced because of the movement.Apoptosis of PC12 cells can induce by H₂O₂,whereas Hovenia dulcis Thunb Water Extract has the antagonism to H₂O₂.The content of quercetin in Hoveniae is quantitatively determined by High Performance Liquid Chromagraphy(HPLC).Hovenia dulcis Thunb extract water contains many flavonoids,and it has the effect of enhance the ability of mice on capacity of cold-resistant,heat-resistant,anti-anoxia and anti-fatigue.The mechanisms probably are related to its activity of scavenging free radicals, improvement of anti-oxidases and the glycogen storage increasing.Studies have shown that Hovenia dulcis Thunb extract water could protect oxidative from damage significantly and had anti-fatigue effect.Hovenia dulcis Thunb extract water can be produced with the form of tablet.