Effect Of Helicobacter Pylori On Cell Proliferation, Apoptosis And Expression Of Nuclear Transcription Factor NF-κB Of Cholangiocarcinoma Cells In Vitro

Purpose: To discuss the influence on proliferation and apoptosis of human bile duct carcinoma cell by Helicobacter pylori (Hp); and signal transduction pathways in cells of this influence.Methods: Apply the CCK-8 (Cell Counting Kit-8) method and flow cytometry to test the influence on proliferation and apoptosis of bile duct carcinoma cell by Helicobacter pylori and cell cycle distribution, utilize the immunocytochemistry and gel electrophoretic mobility shift assay ( EMSA)to analyze the expression and activation conditions of nuclear transcription factor NF-KB before and after Hp. And then use the specific NF-KB inhibitor pyrrolidine dithiocarbamate (PDTC) to inhibit the activation of NF-KB, and utilize the cellular immunity chemical technology and RT-PCR semi quantitative to analyze and compare the expression conditions of anti-apoptosis protein Bcl-2 and Bcl-XL before and after deactivation of NF-KB.Result: At MOI (concentration ratio of bacteria to cell) of 0.5:1, Hp could facilitate the proliferation of QBC939 cells, the breeding ratios on the first and third day are 15.24%±4.2% and 34.18%±2.6% respectively, t test, P<0.05, having statistical significance. There is no induced Apoptosis at this MOI value, but could promote the development of cell cycle; the cell NF-KB is activated and the expression of anti-apoptosis protein Bcl-2 and Bcl-XL is up-regulated; the PDTC inhibition test proves that the expression of anti-apoptosis protein Bcl-2 and Bcl-XL is controlled by the activity of NF-KB.Conclusion: Helicobacter pylori--nuclear factor NF-KB--anti-apoptosis protein Bcl-2, Bcl-XL, the activation of this pathway is likely to play a very important role in the proliferation, apoptosis and malignant transformation of bile duct carcinoma cell.