The Diversity Of Different Types Of Cells In Response To Carbonyl Stress

Senescence is a collective consequence of both inheritance and environment.According to the carbonyl stress theory of aging,the reactive carbonyl species orginated from lipid peroxidation and glycation can readily attack almost all biomacromolecules in vivo,and the crosslink of biomacromolecules induced by carbonyl stress is the essential process and even biochemical mechanism of aging.With the aim to detect the diversity of response to carbonyl stress of different cells,this thesis explored the different effects of carbonyl stress on the erythrocyte and Saccharomyces cerevisiae(yeast) because the viability of erythrocytes in vitro is very weak while the yeast is a living single-cell organism in vitro..Firstly,the blood viscosity will increase with prolonged storage times,while the detailed biochemical mechanism of it remains only partly understood.To study the mechanism,the alteration of thiols,TBARS, carbonylation and fluorescence intensity of erthrocyte membrane proteins were determined.The results showed that,accompanied by blood viscosity increase,the thiols and the protein fluorescence intensity decreased;however,the level of TBARS and protein carbonylation increased singnificantly.These findings indicated that the reactive carbonyl species can attack the amino and sulfydryl residue readily,and the carbonyl stress may be play an important role in the hemorheological alterations during blood storage.Secondly,aiming to detect the response of yeast to carbonyl stress, the yeast was treated with diverse concentrations of MDA.After the yeast was overnight incubated,the optical density at 600 nm of was measured with UV/Vis spectrophotometer,and the viability was assayed with fluorescent spectrophotometer at Ex530/Em590 after stained by PI fluorescent dyes.The results showed that the growth and the viability of the yeast were not affected significantly even at the high concentration of MDA.These findings indicated that the anti-carbonyl stress activity of the yeast was very strong,and the mechanism of it needed further investigation.In summary,the carbonyl stress could cause a series of physiological and biochemical changes to the erthrocytes,however,its had rarely effects on the yeast.