Experimental Studies On The Anti-fatigue And Antioxidant Effects Of Traditional Mongolian Herbs Tamir In Mice

To study and explore the anti-fatigue and antioxidant effects of new preparation of Mongolian traditional medicine-Tamir, the improved preparation based on Narenmandula, the anti-fatigue and anti-oxidation test in mice were carried out respectively in this paper. 48 Kunming mice were randomly divided into 4 groups of 12 in each in anti-fatigue test: groupâ… as a negative control, administered carboxymethyl cellulose(0.5%CM) by per os; groupâ…¡as a positive control, administered Competitor capsule of 0.48g/kg by per os; groupâ…¢and groupâ…£was were groups of Narenmandula and Tamir preparation, administered of 2.07g/kg Narenmandula and Tamir by per os, respectively. And then the time of forced swimming, hypoxia survival, heat resisting survival, contents of the blood Lactic Acid and glycogen in mice were observed respectively. 36 Kunming mice were randomly divided into 3 groups of 12 in each in Anti-oxidation experiment: groupâ… as a negative control; groupâ…¡was Narenmandula and groupâ…¢was Tamir. The liver, kidney, brain and skeletal muscle were sampled immediately finished 10min forced swimming test after 30min of last administratioen, and the activity and content of Total superoxide dismutase(SOD), Catalase(CAT), Glutathione Peroxidase(GSH-PX), total antioxidan tcapacity(T-AOC) and malonaldehyde( MDA ) were determined as soon as possible.The experimental results showed that Mongolian traditional herbs Tamir preparation can significantly prolong the time of forced swimming, hypoxia survival, thermal survival in mice, can decrease significantly the content of lactic acid, significantly increase glycogen content of liver and muscle. Tamir also can significantly decrease the content of MDA in liver, kidney, brain tissue and skeletal muscle, and can enhance the activity of SOD, CAT, GSH-PX and T-AOC in brain tissue and skeletal muscle, however, Tamir has no consentaneous effects on these enzymes activity in liver and kidney. Therefore, Mongolian traditional herbs Tamir preparation can accelerate the elimination of fatigue, can postpone the fatigue and possess strong antioxidant effects in brain tissue and skeletal muscle.