A Related Research On The Changes Of TNF-α And IL-6 In Rat's Blood Serum And Brain Pathology With Sleep Deprivation Under Hypoxia And Hypothermia

Objective:It researches on the concentration of TNF-αand IL-6 in blood serum level and pathological changes in brain of sleep deprivation in Sprague-Dawley rats under hypoxia and hypothermia conditions,and probes into the relationship between the TNF-αand IL-6 and sleep deprivation under hypoxia and hypothermia conditions and the effect on the organism,in order to provide a new theoretical basis about pathogenesis of plateau sleep disorders,comprehend and resovel the issue of high altitude sleep,improve the sleep quality and overall level of health with highland populations.Methods:Healthy adult Sprague-Dawleys were selected at random in five groups. There are 5 Sprague-Dawley rats in each group.The five groups include antitheses group(CC group for normal and TC group for environment) and sleep deprivation group(SD1d,SD4d,SD7d) under hypoxia and hypothermia conditions.The research establishes sleep deprivation model.Having deprived of sleep at different time,the blood and brain were taken out.ELISA(Enzyme-linked immunosorbent assay) were used to test the concentration of the blood serum of TNF-αand IL-6.The brain tissue was sliced and stained in HE.Then the author observes the pathological changes with microscope.All the results are analyzed by SPSS13.0.Results:ELISA test indicates that under hypoxia and hypothermia conditions the concentration of blood serum TNF-αtends to rise as the sleep deprivation prolongs while the concentration of blood serum IL-6 tends to rise first and then decline as the sleep deprivation prolongs.But the concentrations of blood serum TNF-αand IL-6 are higher than that of the CC and TC.The HE staining of the brain shows that the brain tissue edema tends to intensify,the glial cells tend to proliferate dramatically,the blood vessels tend to engorge noticeably and few neuronal degeneration and cytoclasis as the sleep deprivation prolongs under hypoxia and hypothermia conditions.Conclusions:①The expression of TNF-αand IL-6 in blood serum and the pathomorphology of the brain tissue changed as the sleep deprivation of SD rats under hypoxia and hypothermia conditions prolonged,the concentration of blood serum TNF-αtends to rise as the sleep deprivation prolongs while the concentration of blood serum IL-6 tends to rise first and then decline as the sleep deprivation prolongs.But the concentrations of blood serum TNF-αand IL-6 are higher than that of the CC and TC.②The HE staining of the brain shows that the brain tissue edema tends to intensify,the glial cells tend to proliferate dramatically,the blood vessels tend to engorge noticeably and few neuronal degeneration and cytoclasis as the sleep deprivation prolongs under hypoxia and hypothermia conditions.③The level of TNF-αand IL-6 in the blood serum of SD rats and the histopathological chang of cerebrum were related to the degree and duration of hypoxia,hypothermia and sleep deprivation.④The rise in TNF-αand IL-6 caused by hypoxia,hypothermia and sleep deprivation might possibly protect the brain cells in the short run.But long-term continued increase of their level would likely injure the neuron.