| Objective:The study aimed to explore the effects of acupuncture on insomnia by observing the changes in Sleep-Wake cycles and dailyactivities of Sleep-deprived mice intervened with different time points of acupuncture.Methods:Sixty SPF-grade 8-week-old male C57BL/6J mice were randomly divided into five groups: Blank Control group(Control),Self-Recovery group(Recovery),Simulated massage group(Sham),Morning massage intervention group(STM),and Evening massage intervention group(STN).After 4 days of Sleep deprivation and corresponding interventions,the dailyactivity of the mice was monitored for 7 consecutive days using free activity wheels,and the changes in various indicators of the Sleep-Wake cycle,such as time proportion,mean duration,occurrence frequency,and transition times of each phase,were analyzed by recording electrophysiological signals using electroencep HAlography(EEG)for 7 consecutive days.Results:General observation: Before modeling,the fur of the mice in each group was black,shiny,and in good spirits.After sleep deprivation,the fur of the mice became dry,dull,and yellow,and they showed indifference and a tendency to lie down.After corresponding intervention for 7 days,the fur color of the mice in each group did not fully recover,but their mental state improved to some extent.dailyactivity: After sleep deprivation modeling,the dailyactivity of the mice in each group significantly decreased(P <0.01).After corresponding intervention,the Recovery group on the 7th day,the Sham group on the 4th day,the STM group and the STN group on the 2nd day,all recovered to no statistically significant difference compared to the Control group(P> 0.05).Electrophysiological situation:(1)Time proportion: After sleep deprivation,the proportion of Wake phase in each group of mice significantly decreased(P <0.01),the proportion of NREM phase significantly increased(P <0.01),and except for the STM group,the proportion of REM phase significantly increased(P <0.01).After corresponding intervention,the proportion of Wake phase in the Recovery group,Sham group,STN group on the 7th day and the STM group on the 3rd day,all recovered to no statistically significant difference compared to the Control group(P> 0.05);The proportion of NREM phase in the Recovery group,Sham group,and STN group on the4 th day and the STM group on the 3rd day,all recovered to no statistically significant difference compared to the Control group(P> 0.05);The proportion of REM phase in the Recovery group on the 4th day,the Sham group and STN group on the 2nd day,all recovered to no statistically significant difference compared to the Control group(P>0.05).(2)Average duration: After sleep deprivation,the average duration of Wake phase in the Recovery group,Sham group,and STN group significantly decreased(P <0.01),and the average duration of NREM pdase in the Recovery group and Sham group significantly increased(P <0.01),while the duration of other pdases did not show statistically significant differences(P> 0.05).After corresponding intervention,the duration of Wake phase in the Recovery group on the 6th day,Sham group on the 5th day,and STN group on the 2nd day,all recovered to no statistically significant difference compared to the Control group(P> 0.05);The duration of NREM phase in the Recovery group and Sham group on the 4th day,both recovered to no statistically significant difference compared to the Control group(P> 0.05).(3)Number of occurrences: After sleep deprivation,the number of occurrences of Wake phase significantly decreased in each group(P <0.01),while the number of occurrences of REM phase significantly increased in the Recovery group,Sham group,and STN group(P <0.01),and there were no statistically significant differences in other phases(P>0.05).After corresponding intervention,all groups recovered to no statistically significant difference compared to the Control group(P> 0.05)on the 2nd day in the WAKE and REM phase.(4)Transition times: After sleep deprivation,the WAKE-NREM and NREM-WAKE transition times in the Recovery group and Sham group significantly decreased(P <0.01),and the WAKE-NREM transition time in the STN group also significantly decreased(P <0.01);the NREM-REM and REM-NREM transition times in the Recovery group,Sham group,and STN group significantly increased(P <0.01),while only the NREM-REM transition time in the STM group significantly increased(P <0.01).After corresponding intervention,the Sham group recovered to no statistically significant difference compared to the Control group(P>0.05)on the 3rd day in the WAKE-NREM transition,and all other groups recovered on the 2nd day.Conclusion:(1)After sleep deprivation,the dailyactivity of mice decreased,and the sleep-wake cycle was disrupted,manifested as a decrease in WAKE phase in terms of time proportion,duration,and number of occurrences,and an increase in NREM and REM phases.The dailyactivity and sleep-wake cycle of mice can recover to normal levels after 7 days.(2)The effect of Sham massage is similar to that of self-recovery,but it is better than self-recovery in improving the proportion of REM phase and the duration of WAKE phase.This suggests that gentle stimulation may have a certain improvement effect on the sleep-wake cycle after sleep deprivation.(3)The Du Meridian massage can intervene in the sleep-wake cycle by increasing the proportion and average duration of WAKE phase,decreasing the proportion and average duration of NREM phase,and decreasing the proportion of REM phase.This suggests that Du Meridian massage may alleviate sleep pressure and improve sleep stability.(4)In terms of improving the proportion of different time phases and the duration of WAKE phase,the effectiveness of morning massage therapy on mice is superior to that of evening massage therapy.This suggests that massage therapy before sleep may have a better effect on improving the sleep-wake cycle of insomnia patients. |
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