| Objective:1. Set up in diabetic rats and diabetic cataract model, diabeticrats and to explore the mechanism of cataract.2. To observe the formation of diabetic cataract in the lens opacities Change and related biochemical changes.3. To explore effect & mechanism the water decoction of the chain purslane urine streptozotocin (STZ)-induced diabetic cataract in rats.Methods:Model before the randomly selected 10 healthy rats as normal control group (A group), and then streptozotocin (STZ, Sigma Company)-induced diabetic rats were randomly divided into 4 groups: Decoction high water purslane dose treatment group (B group, 1.662g/ml), Portulaca oleracea decoction dose treatment group (C group, 0.831g/ml), Portulaca oleracea decoction of low-dose treatment group (D group, 0.415g / ml) and diabetes mellitus model group (E group). Drencher 1 times daily, every two weeks to monitor blood glucose, body weight measured each week and to observe the crystal changes, and record. Model after 9 weeks, were determined lens superoxide dismutase (SOD), malondialdehyde (MDA), water soluble protein (WSP) content.Results:1. During the experimental process, the normal rat lens has always been transparent.After STZ injection 3wk, beginning in diabetic rats lens opacification happened, the first 3 weeks to 6wk progress was slow, but after 6wk progress was rapid. The degree of lens opacification in diabetic rats significantly heavier than normal rats, purslane water decoction significantly slowed in diabetic rats lens opacification. After STZ injection at 3w-section at the end 7w, high, medium dose group rat lens turbidity lighter than the model group, the degree of lens opacity (P<0.05); high, medium dose group rat lens turbidity light lens in low-dose group turbidity (P<0.05); at STZ injection at different time points after high-dose group and middle dose rats crystalline considerable progress turbidity (P>0.05).2. Biochemical indicators of test results: STZ injection after 9wk, normal control rats as the standard, the model group compared with normal control group, MDA was significantly increased (P<0.05), SOD activity and soluble protein content decreased significantly (P<0.05); the treatment group in each group significantly decreased MDA content, enhance SOD activity, compared with the model group were significantly different (P<0.05). Among them, the high dose group rat lens MDA lower DM content, the enhance role of SOD activity was better than in the low dose group (P<0.05). In the low dose group of DM rats compared the effects of crystal no significant difference (P>0.05).Conclusion:1. STZ-induced diabetic rats and diabetic cataract model can be used to study diabetic cataract;2. STZ-induced DM rat lens antioxidant enzymes within the SOD activity decreased MDA content of oxidation products increased; simultaneously reduced soluble protein on the pathogenesis of diabetic cataract mechanism and oxidative damage.3. Purslane decoction can inhibit the early occurrence of diabetic cataract and development, its mechanism and its possible to improve rat lens in the activity of antioxidant enzymes SOD, MDA oxidation products to reduce production, reduce the degree of crystal oxidative damage, effectively prevent the soluble protein to insoluble protein to change. |
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