| ObjectInfluenza virus can cause respiratory tract infections, incidence of acute and widespread popularity,spread rapidly,the elderly,chronically ill children and vulnerable groups of infection complications and high mortality.As the influenza virus surface antigen to continuous antigenic drift occurs and conversion, so that an effective vaccine would have soon expired,making influenza pandemic constantly on people's physical health at risk. Influenza virus-induced respiratory tract infection when the line belongs to Chinese medicine the scope of a cold temperature and wind. XiaoChaiHu Decoction in the "Shanghan theory" Shaoyang reconciliation on behalf of the party.Fangzhong to weihan of Chaihu kuxin for King,half tables qingshu Shaoyang evil to the bitter cold for Hill Baicalin,money spilled half of the evil Shaoyang,the drug played a total match of reactive Shaoyang reconciliation. Accompanied by Banxia,ginger and antiemetic Hewei Jiangni;ginseng, jujube Jianpi Yiqi;Zhigancao help Senate,Fuzheng date,and to reconcile the various drugs to make.Cold and heat and all-side attack and make up facilities,the coordination of movements,there is reconciliation Shaoyang,Shu-li Sanjiao,regulation of gas machine,inside and outside Qualcomm announced,the role of Cardinal running.Drug alone and combined accurate Qiwei,set of rigorous,effective,and safe and reliable.Xiao Chai Hu Decoction study mechanisms regulating the immune mechanism against viral infection,as well as the combined group synergy mechanism revealed by the combination treatment side and the scientific rigor of traditional Chinese medicine to promote and discover the treasure house of traditional Chinese medicine guide clinical treatment,are of great significance.MethodsThis experiment by simulating the human respiratory virus infection,replication of a respiratory infection mouse model experiment,be divided into different groups to ribavirin as a control group of western medicine,through the whole side of Xiao Chai Hu Decoction and Chai Qin,Jiang Xia,Senate grass Calligonum, Chai Qin+Jiang Xia,Chai Qin+Participation date down to grass and other treatment,the use of modern immunology and other biological sciences advanced technology used to observe the dismantling of Xiao Chai Hu Decoction's role and its mechanism,and explore Xiao Chai Hu Decoction "Fuzhengquxie" and "and the" nature of the basic law to seek compatibility.Specific methods are as follows:1,through the two viruses to adapt to the process of rat lung, and then inoculated by the chick embryo cysts passage,preparation of new drugs. 2,determination of virus titer in mice,the virus LD50,for the use of mice in vivo.3,the mice were divided into 9 groups,the model and after treatment,using precision electronic weighing scales weight and lung weight in mice,lung index and lung index calculation of inhibition rate,to observe and demolition of Xiao Chai Hu Decoction on influenza virus infection of the protective effect in mice.4,the mice were divided into 9 groups,the model and treatment, eye blood,serum,using double-antibody sandwich ELISA method in sera of mice in each group of tumor necrosis factor-α(TNF-α) and interleukin-2(IL-2) expression levels,observation of Xiao Chai Hu Decoction and split influenza virus infection on immune modulation in mice.Result1,Xiao Chai Hu Decoction Fang-wide group,Chai Qin group,Chai Qin+ginger summer group and Chai Qin+Participation date of lung grass group index compared with control group P＜0.05,indicates that with Xiao Chai Hu Decoction and Radix,baicalin all demolished in order to reduce influenza virus infection in mouse lung index, indicating that its demolition of Xiao Chai Hu Decoction on influenza virus infection in mice have protective effects,and to the best all-party group.2,compared between different treatment with group the concentration of TNF-α,we can conclude:the blank control group was significantly higher than the normal group,the difference was statistically significant(P＜0.01),prompted a 1-type of influenza virus infection in mice after the release of TNF-αincrease in the body may be caused by inflammatory injury;Xiao Chai Hu Decoction Fang-wide group,Chai Qin+Participation date and reference date grass grass mice serum TNF-αthan the control group,the difference was statistically significant(P＜0.05),prompted the group to reduce the serum of mice infected with influenza virus as a result of the increase in TNF-αcontent,which may reduce the inflammatory response;ribavirin group,Chai Qin group,Chai Qin+Jiang Xia Jiang Xia and serum TNF in mice-αalso decreased,but the model group and the difference was not statistically significant.3,compared between different treatment group the concentration of IL-2,we can see:the blank control group,serum IL-2 lower than the normal group,the difference was statistically significant(P＜0.01),prompted a 1-type of influenza virus can lead to IL-2 significantly decreased immune function in mice affected; ribavirin group than the control group,the difference was statistically significant(P＜0.01);Xiao Chai Hu Decoction group, Chai Qin group,Chai Qin+Jiang Xia group,Chai Qin+Participation date grass mice serum IL-2 was significantly higher than the control group,the difference was statistically significant(P＜0.01), prompted the group of mice infected with influenza virus due to the decline in immune function and significantly improve the effect; Participation date summer grass and ginger mice serum IL-2 also have increased,but the model group and the difference was not statistically significant.ConclusionDown side is the study of Chinese herbal compound used in the study group theory to the most commonly used method,the use of this research to a deeper understanding of compound compatibility law of traditional Chinese medicine.Comprehensive results of this experiment,Xiao Chai Hu Decoction significant role in the anti-influenza virus,and can effectively prevent the excessive inflammatory response of the body caused by autoimmune injury,its role is better than the down side;in Xiao Chai Hu Decoction prescription in order to Chai Qin compatibility of each group of anti-influenza virus compound the role of the strongest,indicating that the prescription,the principal component Chaihu,Baicalin in the anti-influenza virus infection may play a major part of the role, and ginseng,jujube,licorice may be the main play a role to enhance the body immunity.Xiao Chai Hu Decoction for clinical applications and further research to provide a certain amount of experimental evidence. |
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