The Serum Levels Of IGF-1, IGFBP3, GHBP And The Plasma Level Of Ghrelin In Short Stature Children And Their Relationship With Growth

Etiologic and the serum levels analysis of IGF-1,IGFBP3 in 164 short stature childrenObjective:To explore the etiology and the serum levels of IGF-1,IGFBP3 in 164 short stature children.Methods:164 short stature children were collected,among them 109 were males(66.5%), 55 were females(33.5%),the average age was(8.24±2.54) years,and 20 controls.All of the children were collected detail medical history and taken both physical and laboratory examination,and we measured the serum levels of IGF-1,IGFBP3 in partial short stature children.The results were analyzed by t-test and one-way ANOVA.Results:86 cases(52.4%) were diagnosed as idiopathic short stature(ISS),including constitutional delay of growth and puberty(CDGP) and family short stature(FSS);57 cases (34.8%) were diagnosed as growth hormone deficiency(GHD).Serum IGF-1 and IGFBP3 levels in children with CGHD were significantly lower than control(75.6±25.3ng/ml vs 158.6±65.3ng/ml,879.1±391.4ng/ml vs 2304.5±498.6ng/ml,P＜0.01,respectively); there was no statistical difference between the PGHD and ISS group(102.4±57.5 ng/ml vs 123.6±58.8ng/ml,1495.1±624.3 ng/ml vs 1845.6±654.9ng/ml,P＞0.05,respectively).15 cases(9.1%) were diagnosed as intrauterine growth retardation(IUGR);6 cases(3.7%) were diagnosed as Turners syndrome.Conclusions:Idiopathic short stature is a common disease among them,and the second is growth hormone deficiency(GHD),determining serum IGF-1 and IGFBP3 levels could be applied for screening and diagnosis of CGHD.There was a partial overlapping between PGHD and ISS,deservering of further study.The serum levels of IGF-1,IGFBP3,GHBP and the plasma level of ghrelin in short stature children and their relationship with growthObjectives:To explore the levels of IGF-1,IGFBP3,GHBP and ghrelin in short stature children and their roles on the growth.Methods:A group of 70 short stature children and 20 age matched healthy children were collected.The short stature children were devided into 3 sub-group according to the different GH peak of GH stimulation test:＜7ng/ml,7-10ng/ml,＞10ng/ml,and were named growth hormone deficiency1(GHD1),growth hormone deficiency2(GHD2) and idiopathic short stature(ISS).All of the children were collected detail medical history and physical examination.We measured the serum levels of IGF-1,IGFBP3,GHBP and the plasma level of ghrelin in all group.And the results were analyzed byχ~2-test,one-way ANOVA, pearson correlation test and multiple regression analyses.Results:1.The height of GHD1 is the most delayed,and statistical difference is existed between the GHD1 and the control(113.60±9.78cm vs 126.78±11.84cm,P＜0.05).The height between GHD2 and ISS is similar(118.51±12.71cm vs 116.79±14.68cm,P＞0.05).There was no statistical difference between the GHD1 and ISS group((113.60±9.78cm vs 116.79±14.68cm,P＞0.05).2.The levels of serum IGF-1 in GHD1 group were lower compared with the other groups, IGFBP3 levels of the group were similar(P＜0.05).The levels of serum IGF-1 and IGFBP3 in GHD2 group were similar to the ISS group.There was no statistical difference in the GHD2 or ISS group compared to control. 3.The levels of plasma ghrelin in GHD1 group were lower compared with the other groups(P＜0.05).The levels of plasma ghrelin in GHD2 group were similar to the ISS group,higher compared with the control group(P＜0.05).There was a positive correlation between plasma ghrelin and serum IGF-1 in GHD1 group(r=0.89,P＜0.05).In the multiple regression analyses,weight was the independent determinant of ghrelin(β=-188.46,P＜0.0001).4.The levels of serum GHBP in GHD2 group were similar to the ISS group,lower compared with the control groups(P＜0.05).There was no statistical difference between the GHD1 and control groups(P＞0.05).5.After adjusting weight for ghrelin as the covariate,ghrelin were found to be similar between groups(P＞0.05).Conlusions:1.There was statistical difference between GHD1 and control group in both physical and laboratory examination.There were great similarity in GHD2 and ISS groups. Combining GH provocative test and serum IGF-1,IGFBP3 levels may serve as an useful parameter in the diagnosis of GHD whose GH＜7ng/ml.2.The results of our study suggest that ghrelin plays a role as a cause in GHD1 group,while as a compensatory or existing ghrelin resistance in the group whose GH＞7ng/ml in the GH provocative test.3.The levels of serum GHBP were lower in GHD2 and ISS group.It may suggest the possibility of GHR mutation.