The Changes Of Serum Levels Of Ghrelin And IGF-1 In Idiopathic Short Stature Children And Their Relationship With Helicobacter Pylori Infection

Objective: The aim of this study was to investigate the prevalence of helicobacter pylori(HP) infection among aged 2 to 15-years-old children who have gastrointestinal symptoms and to compare the incidence of short height in the children who infected helicobacter pylori or uninfected helicobacter pylori in order to find the impact of helicobacter pylori infection on children's growth.Methods: with gastrointestinal symptoms or short stature six hundreds and four children aged 2 to 15-years-old, who tested H pylori by the 13C-urea breath test, form Children's Hospital Affiliated to soochow University to seek treatment in 2010-9 to 2010-12. Among them 324 were males(53.6%), 280 were females(46.7%), the average age was (7.80±2.79)years. All of the children were collected detail medical history and taken both physical and laboratory examination, and we measured the height of these children and calculated the incidence of short height. The results were analyzed by t-test and chi square-test.Result: Mean prevalence of HP infection in aged 2 to 15-years old children with gastrointestinal symptoms was 31.6%; the prevalence was 32.1% in male and 31.0% in female. The prevalences of Hp infection among different age groups were as follows: 7 years, 27.1%, 8 year, 29.4%, 9 year, 29.7%, 10 year, 30.6%, 11 year, 32.5%, 12~15 year, 43.5%. The incidence of short height in infected H pylori children(25.1%) were higher than those (19.6%)who uninfected H pylori. And the incidence of short height in children aged 7 to 15 were higher than those aged 2 to 6.in the group with HP infected( 28.1% vs 20.8%,P<0.05), with significant difference.Conclusions: 1.The prevalence of HP infection in aged 2 to 15-years old children with gastrointestinal symptoms was 31.6% and it increased with age; 2.Infected H pylori can impact on children growth. So we should test H pylori by the 13C-urea breath test or serum HP-IgG antibodies for those children with gastrointestinal symptoms or short stature and take comprehensive treatment.Part II The changes of serum levels of Ghrelin and IGF-1 in idiopathic short stature children and their relationship with helicobacter pylori infectionObjective:To measure the serum levels of ghrelin and IGF-1 in idiopathic short stature(ISS) children and controls, explore the relationship between the change of serum levels of Ghrelin and IGF-1 and helicobacter pylori(H pylori) infection.Methods: We collected a total of 86 children with gastrointestinal symptoms or short stature form Apr.2010 to Feb.2011 who came to Children's Hospital Affiliated to soochow University. According to their height, all of them were devided into 2 sub-group and the short stature group need to take growth hormone stimulate test. And then we chose 47 idiopathic short stature children whose GH peak of GH stimulation test>7ng/ml, among them 36 were males, 11 were female, the average age was (9.34±3.23)years; The control group were selected 39 age,gender matched children with normal height, among them 21 were males, 18 were female, the average age was (8.40±1.68)years. And according to the 13C-urea breath test result, all of them devided into HP infected group 43 children and HP uninfected group 43 children. All of the children were collected detail medical history and taken both physical and laboratory examination. we measured the serum levels of IGF-1, Ghrelin in all group. And the results were analyzed by Kruskal Wallis H test, t-test and Pearson Correlation test.Result: 1.serum ghrelin levels(242.91±82.46ng/l vs 286.26±96.53ng/l )in children with helicobacter pylori infection were lower than those without helicobacter pylori infection, with significant difference(P<0.05). And serum ghrelin levels (303.05±80.31ng/l vs 205.96±60.68ng/l) in idiopathic short stature children were higher than normal height subjects, with significant difference(P<0.05); Among the ISS, the serum ghrelin level in chidren with HP infected were lower than those with out HP infected(285.79±77.20 vs 336.12±84.06, P<0.05); 2.serum IGF-1 levels in ISS children and normal height children have no statistically significant difference(142.50±75.67ng/ml vs 148.58±65.60ng/ml P>0.05). It also have no statistically significant difference between children with HP infected and children without HP infected(160.09±82.73ng/ml vs 130.42±53.73ng/ml P>0.05). 3.There was a negative correlation between serum ghrelin and height,weight.Conlusions: 1.The serum ghrelin levels were found to be significantly decreased in children with Helicobacter pylori infection. 2. The serum levels of ghrelin are higher in ISS children than normal children. The increasing of serum ghrelin maybe is only a compensation performance of low weight and short stature or is possible a mechanism of resistance to ghrelin increasing which GHS-R deletion or mutation in ISS children lead to ghrelin can't combined with GHS-R normally. 3.The surem level of ghrelin has negatively correlated with weight and height.