The Changes Of Activity And Clinical Significance Of Serum Paraoxonase One In Nonalcoholic Fatty Liver Disease Patients With Metabolic Syndrome

Objective:To investigate the changes of activity of serum paraoxonase one(PON1) and the degree of oxidative stress for clarifying an role and clinical significance both serum PON1 and oxidant stress in nonalcoholic fatty liver disease(NAFLD) with metabolic syndrome and the NAFLD.Methods:26 cases of NAFLD patients with metabolic syndrome(MS) and 38 cases of the patients with NAFLD and 26 cases of check up individual from Mar 2003 to Dec 2008 consultation in the First Hospital affiliated Ji Nan University were enrolled in the study.The NAFLD with MS was a trial groups,the NAFLD and check up individual were as control groups.Abdominal ultrasound was taken for fatty liver examination. Body height,weight,waistline,BMI,and blood pressure were measured.Biochemical assay was used to determine FBG,ALT,GGT,TC,TG,HDL-C,LDL-C,PON1,MDA,SOD and so on.The changes of activity of serum PON1 and the oxidant metabolic reaction were observed,meanwhile the relationships between serum PON 1 and clinical biochemical indicators in different groups were also analyzed.Results:1.The activity of serum PON1 and the level of SOD were significantly decreased,the level of MDA was obviously increased in NAFLD patients with MS compared with the NAFLD patients and the controls (p＜0.05 or p＜0.01).The activity of serum PON1 and the level of SOD were decreased and the level of MDA were increased in the NAFLD patients compared with the normal controls(p＜0.05 or p＜0.01).2..Serum PON1 was correlated positively with SOD(r=0.892,p＜0.01),and negatively with ALT,AST, GGT,MDA(r=-0.835,r=-0.574,r=-0.465,r=-0.828,p＜0.01),and was not correlate with BMI(r= -0.339,p=0.058),Waistline(r=-0.153,p=0.402),FBG(r=-0.338,p=0.058),TC(r=-0.229,p=0.208),TG (r=-0.224,p=0.178),HDL-C(r=-0.298,p=0.097),LDL-C(r=-0.015,p=0.934) in the MS group. Multiple regression analysis was shown that SOD,MDA,ALT in the MS groups were linear correlation with activity of serum PON1.3.Serum PON1 was correlated positively with SOD(r=0.872,p＜0.001),and negatively with ALT,AST, GGT,MDA(r=-0.761,r=-0.493,r=-0.521,r=-0.816,p＜0.01),and was not correlate with BMI(r=-0.246, p=0.136),Waistline(r=0.271,p=0.100),FBG(r=-0.253,p=0.125),TC(r=-0.254,p=0.123),TG(r=-0.205,p= 0.218),HDL-C(r=0.141,p=0.397),LDL-C(r=-0.156,p=0.348) in the NAFLD with MS group.Multiple regression analysis was shown that SOD,MDA,ALT in the NAFLD groups were linear correlation with activity of serum PON1.4.Serum PON1 was not correlate with the clinical biochemical indicators in normal groups.Conclusion:1.Activity of serum PON1 was one of scout indicator in the degree of oxidative reaction and liver functional damage for the patients of NAFLD with metabolic syndrome and NAFLD.2.the change of the serum PON1 combined with MDA and SOD were assayed as a surveillant index in the evolutive course of NAFLD with MS and NAFLD.