Screening For Effective Part Of Activating Blood Circulation In Fo-Shou Powder And HPLC Fingerprint Of Rabbit Serum After Administration Of Effective Part

Objective:By the establishing the serum fingerprint and pharmacodynamics research of the different extract parts in Fo-Shou Powder, screen for the effective parts which can activate the blood circulation.Methods:Fo-Shou Powder were extracted by supercritical C02 fluid and obtained the part of SFE, its dregs that extracted by alcohol were divided into several different parts of the alcohol-eluting(the parts of EE20, EE40, EE60, EE95). The fingerprint of the part of SFE was determined by GC/MS and the fingerprint of several different parts of the alcohol-eluting. Different extracted parts were gavaged to mice that induced acute myocardial ischemia by intraperitoneal injection of isoprenaline, and determined the lift of S~T segment in ECG before and after administration and the membrane fluidity of red blood cells. Different extracted parts were gavaged to rabbits to collect serum at different time points, and established serum HPLC fingerprins to screen for the effective part of activating blood circulation in Fo-Shou Powder combined with anti-myocardial ischemia pharmacodynamic study.Results:Compared with serum HPLC fingerprint of different extracted parts before and after administration, it showed the groups of EE20, EE40, EE60 and SFE were different from blank group;compared with changes of Euclidean Distance before and after administration,it showed the groups of EE20 and EE40 were absorbed fastest. Compared with the control group, it showed fluorescence polarization decreased by means of anti-myocardial ischemia pharmacodynamic study and had a significant difference(P<0. 05, P<0.01), for the effect of the lift of S-T segment, the groups of EE20 and SFE had a significant difference in 5 minutes (P<0.01,P<0.05), the groups of EE20, EE40 and SFE had a significant difference in 10 minutes (P<0. 01, P<0. 05), the groups of EE20 and SFE had a significant difference in 20 minutes (P<0. 05, P<0. 01). Conclusion:The groups of EE20,EE40 and SFE can counteract the increase of AS-T segment induced by Iso, protect myocardial cells so that play an anti-myocardial ischemia role and increase the ability of anti-hypoxic ischemic. From the experiment of erythrocyte membrane fluidity, it showed the groups of EE20 and EE40 also can enhance the membrane fluidity compensatory to improve energy metabolism in period of acute ischemic and prevent further myocardial necrosis. The research of serum HPLC fingerprins of different extracted parts before and after administration will contribute to the discovery of effective parts in order to increase the probability of the discovery of active components and shorten the cycle.