Experimental Research Of Basil Polysaccharide Against The Invasion And Metastasis Of Ovarian Cancer Cell Lines In Vitro

Objective: To investigate the possible mechanism of basil polysaccharide in inhibiting the invasion and metastasis of ovarian cancer,and provide a theoretical basis for finding clinical effective drugs against the metastasis of ovarian cancer ovarian.Methods:The human ovarian cancer cell lines SKOV3 cultured with basil polysaccharide under normoxia(21%O2,5%CO2)and hypoxia(1%O2,5%CO2,94%N2)respectively. Morphology of different groups of this cell line under different oxygen concentration were observed with inverted phase contrast microscope; the apoptosis of human ovarian cancer cell line were tested by cell apoptosis-Hoechst 33258 staining;the proliferation of the cell line was evaluated by MTT assay; the expression of OPN mRNA and protein levels were detected by RT-PCR and Immunol cytochemistry, respectively; the difference of matrix metalloproteinase-2 (MMP-2)'s activity among each group was analyzed by Gelatin zymography and the cell invasiveness was tested by Transwell method.Results: Compared with the control group, basil polysaccharide could promote the apoptosis of human ovarian cancer cell line(SKOV3) and inhibit the proliferation of SKOV3 cell line,but in normoxia the function of basil polysaccharide was opposite;furthermore,basil polysaccharide could reduce the expression level of osteopontin (transcriptional level and protein level)of the SKOV3 cell line ,it could also inhibit the secretion of MMP-2 of SKOV3 cell line and could also decrease the invasiveness and migrating ability of the SKOV3 cell line both in normoxia and in hypoxia,moreover,these functions of basil polysaccharide were more obviously in hypoxia.Conclusions: Basil polysaccharide has the anti-metastasis function of ovarian cancer , the action mechanism is related to the reduced expression of osteopontin, the inhibited secretion of MMP-2 and the decreased invasiveness and migrating ability of the SKOV3 cell line; The oxygen environment may affect the anti-tumor metastasis activity of basil polysaccharide.