Preparation And Evaluation Of PLGA Nanoparticles As Drug Delievery System For Anti-cancer Drug And Peptides

Nanoparticles(NPs) is a very promissing drug delivery system, which possesses many advantages over the traditional drug delivery systems. Polylactide(PLA) and Polylactide-co-glycolide(PLGA) have been applied widely in the control release preparation field. The purpose of this study is to research the drug loaded Polylactide-co-glycolide Nanoparticles (PLGA-NPs), optimizing the technology and evaluating the release behavior in the vitro, which is contribute to clinic application in the future.5-fluorouracil(5-Fu) loaded PLGA-NPs were prepared by improved double emulsion (water-in-oil-in-water, W/O/W) method. The characters of the NPs were measured by SEM, TEM, and the entrapment efficiency and drug loading of 5-Fu loaded PLGA-NPs was investigated via UV method. The results showed that NPs prepared by the best condition were spherical in shape, and the particles diameters ranged from 100-200nm. The entrapment efficiency and drug loading was 36.03% and 10.02% respectively.The release behavior of 5-Fu NPs was investigated via UV method. It was found that the release of 5-Fu from NPs had three periods, i.e., burst release, sustained release and last release period, and the NPs had a good sustained release effect on the 5-Fu.Based on the research on the water-soluble anti-cancer drug loaded PLGA-NPs, the peptide drug delievery system was also studied. BSA loaded PLGA-NPs were prepared, and it showed that the NPs can be also applied to the peptide drug.