The Immunological Mechanism Of Antitumor Efficacy Of GTR On H₂₂ Tumor Bearing Mice

Objective: To study the relationship between antitumor efficacy of GTR and its effect on immunological function in H₂₂ tumor bearing model mice, probe into the immunological mechanism of GTR inhibiting tumor's growth.Methods: The different dose of GTR was douched to H22 tumor bearing model mice by oral, we observed the tumor growth inhibitory rate (IR) and the index of thymus and spleen. We observed the antitumor efficacy of the Serum containing GTR applying serum pharmacology, and measured the level of IL-2 and IL-4 in serum applying ELISA method when we observed the influence of GTR acting on the cytokine. To measure T cell phenotype with FACS method and observe the influence of GTR acting on the CD4⁺,CD8⁺ molecule.Results: The result showed that the tumor growth inhibitory rate( IR) of GTR has obviously different (P<0.01) Compared with the model group, the tumor growth inhibitory rate is 39.14%. The Serum containing GTR can inhibit the growth of tumor, but the tumor growth inhibitory rate is lower than in body. The index of thymus,the IL-2's level and the CD4⁺'s value of the group of GTR were higher than it in the model group (P<0.01) .Conclusion: The GTR has antitumor effect, the proper dose is 825mg.kg^-1.d^-1, the tumor growth inhibitory rate is 39.14%. GTR can regulate thysum growth. GTR can regulate cytokine to correct the phenomenon of T_H1/T_H2 drift in tumor mice, maintain T_H1 cytokine of advantage position and correct the disbalance of CD4⁺/CD8⁺ to normal. The immunosuppressive state of the tumor mice was regulated.