Studies On Polygona-polysaccharose's Effects Upon APP Transgenic Mice's Learning Ability & Memory And The Ultrastructure Of Hippocampus's CA1 Region

Alzheimer's disease (AD) is the degeneration of the central nervous system (CNS). It's clinically characterized by impairment of memory & cognition, disordered behavior and changed personality, while Neurofibrillary tangles (NFT) ,βAmyloid protein deposition, Neuronal degeneration or apoptosis, neural sheath solution and vascular amyloidosis in brain tissue are its pathological features. With the aging of the population, AD has become one of the most severe problems, which threaten people's life span and quality of life. So it is of great practical value for us to study the disease.The cause of AD is unknown, and the treatment of it is still in the process of exploring. Polygona-Polysaccharose (PP) is extracted from Rhizoma Polygonati, which is a traditional Chinese medicine that can lengthen one's life. PP can enhance immunity and anti-aging, and so on. In this experiment, we interfered APP transgenic mice with different concentration of PP, and the purpose is to explore the effects and functions of PP on APP transgenic mice, thus to find a new way to prevent and treat the experimental AD.Objective: To investigate the effect and function of PP on APP transgenic mice.Method: Inject different concentrations of PP solution into the mice's stomach for 45 consecutive days. The Morris water maze was adopted to test the ability of the mice's initiative learning ability and memory; Transmission Electron Microscope (TEM) was used to observe the ultrastructure of hippocampus; Stereology was adopted to analyze the ultrastructure index and SPSS13.0 was used to analyze the above-mentioned data.Result: The results show that PP has the following effects:1. Ethology: Compared with the blank group, the delitescence of the treated group shortened obviously, the frequency of finding the platform increased, and the frequency of making mistakes decreased (p<0.01). Within the treated group, the group with high concentration PP increased in the frequency of finding the platform, shortened in the delitescence, and decreased in the frequency of making mistakes (p<0.05).2. Hippocampus' ultrastructure: Compared with the blank group: (1) The number of the neurons in the treated group increased while the degeneration degree of the neurons relieved; (2) Mice's brain tissue edema and vacuoles degeneration decreased obviously in the treated group, there are more obvious effect in the high-dose PP group(p<0.01). The stereology parameters of mitochondria, synapse and synaptic interface structure have improved obviously in the treated group (p<0.05). There are significant differences among the groups with different concentration of PP (p<0.05).Conclusion: PP can improve APP transgenic mice's central nervous system (CNS) ultrastructure, maintain CNS homeostasis, prevent peroxidation & inflammatory reaction, protect the central cholinergic system, and improve the learning ability and memory. There are dose-effect relationships. PP is an effective prescription for the prevention and therapy of experimental AD.