Study On Hypoglycemic Effect Of Polygona-polysaccharose On Experimental Diabetic Mice

ObjectiveTo study the effect of Polygona-polysaccharose on blood glucose of normal mice; To study the effect of Polygona-polysaccharose pretreated and treated on blood glucose of diabetic mice induced by alloxan(ALX).MethodsPart 1 The effect of Polygona-polysaccharose on blood glucose of normal mice. The normal mice were randomly divided into control group and PSP(330,660,1320 mg·kg-1). Then the fasting blood glucose(FBG) was detected.After 7 days administration of PSP, the blood glucose was detected for the second time.Part 2 The effect of Polygona-polysaccharose pretreated on blood glucose of diabetic mice induced by alloxan(ALX).1 The mice were pretreated by PSP (330, 660, 1320 mg·kg-1) for 7 days.On the 8th day, we injected Alloxan (ALX, 220 mg/kg) into its abdominal cavity. After 72 hours, the blood glucose was detected. FBG were detected by glucose oxidase method, then NO and NOS in blood serum were detected by colorimetric method.2 After the death of mice by cervical dislocation, removed part of the liver and kidney rapidly, made 10% of the tissue homogenate. The content of NO and NOS of liver and kidney were measured by chemical colorimetric method. 3 Removed the pancrea of mice rapidly in each group, took the same position fixed by 10% neutral formalin, embedded by paraffin, then with conventional HE staining. Finally observed the pathological changes of pancreatic tissue under the microscope.Part 3 The effect of Polygona-polysaccharose treated on blood glucose of diabetic mice induced by alloxan(ALX).1 The ALX (220 mg·kg-1) intra-abdominal injection was used to establish a mouse model of diabetes.72h later, FBG was detected of the mice. When the FBG > 11.1mmol / L ,the mouse was thought to be a model of success.2 Successful models were randomly divided into model group, glibenclamide (2.5 mg·kg-1) and PSP (330,660,1320 mg·kg-1) groups with continuously administration for 15d. Model group and control group ig the same capacity of distilled water.3 24h after the last administration, blood was drawed from eyes and then made separation of serum; Using glucose oxidase method tested FBG of mice; Chemical colorimetric measurement was used to detect TG, TC, NO, NOS of serum and NO, NOS concentration of liver, renal tissue; Check the thymus, spleen index; INS concentration in serum was checked by radioimmunoassay (RIA) measurement.4 Removed the pancrea of mice rapidly in each group, took the same position fixed by 10% neutral formalin, embedded by paraffin, then with conventional HE staining. Finally observed the pathological changes of pancreatic tissue under the microscope4 Immunohistochemical method was used to observe the expression of INS in pancrea of ALX-induced diabetic mice.ResultsPart 1 The effect of Polygona-polysaccharose on blood glucose of normal mice. Experimental results showed that, compared with the control group, the mice which were pretreated by PSP for 7 days, did not significantly affect blood glucose levels. And compared with the their own blood glucose before, PSP groups had no significant difference in blood glucose.Part 2 The effect of Polygona-polysaccharose pretreated on blood glucose of diabetic mice induced by alloxan(ALX).1 Bood glucose of mice in model group significantly increased after injecting ALX (220 mg·kg-1); Preventive given PSP (1320 mg·kg-1) group increased blood glucose a little after injecting ALX,compared with the model group, which was significant. Preventive given PSP (330,660 mg·kg-1) groups after injecting ALX,the blood glucose partly decreased,but compared with the model group, there was no statistical significance.2 NO and NOS were significantly increased in serum of model group; NO and NOS were significantly reduced in mice serum of preventive given PSP (660,1320 mg·kg-1) groups; There was no significant difference in the content of NO and NOS in liver and kidney of the PSP groups3 The results of HE showed that there was no significant difference between preventive given PSP groups and model group in histopathological damage of pancreas.Part 3 The effect of Polygona-polysaccharose treated on blood glucose of diabetic mice induced by alloxan(ALX).1 PSP (330,660,1320 mg·kg-1) could significantly reduced the increased blood sugar of ALX-induced diabetic mice, increase the INS levels in serum and had no significant effect on TG, TC in serum.2 PSP (660,1320 mg·kg-1) could significantly reduce the levels of NO and NOS in serum,liver and NO in the kidneys, but had no significant effect on renal NOS.3 The results of HE showed that, the pancrea in the model group almost could not find complete islet, the islet structural disorderd and the edge was not clear. The islet in PSP (660,1320 mg·kg-1) groups were relative regularity. 4 Immunohistochemistry results showed that in the control group, a large number of scattered islets were clearly observed, and a large number of brown, darker color particles produced. Almost no a clear brown insulin particle was observed in the model group.The amount of brown insulin particles in PSP (660,1320 mg·kg-1) groups increased and the number of scattered islets also increased.ConclusionsPSP did not significantly affect blood glucose levels in normal mice; PSP pretreated had a little protective effects on diabetic mice induced by ALX; PSP treated had some protective effect on diabetic mice induced by ALX, and the mechanism might be related with protecting pancrea, increasing insulin and lowering NO and NOS in blood serum and liver.