Proteomics Analysis Of Hypothalamuses And Adrenal Glands In Hypertensive Rats With Hyperactivity Of Liver-yang With Pinggan-Qianyang Treatment

SUBJECT1. To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from hypothalamuses and adrenal glands of normal rats,hyperensive rats with hyperactivity of liver-yang and the hypertensive rats with hyperactivity of liver-yang with Pinggan-Qianyang treatment.2. To explore the different expression in hypothalamic and adrener-gdrenic proteins of hypertensive rats with hyperactivity of liver-yang with Pinggan-Qianyang treatment, and probe into the hypertension pathogenesy and mechanism of decreasing the blood pressure and ameliorating the symptoms of Pinggan-Qianyang treatment from the level of proteomics, and provide theories evidences for new medical marked protein.METHODS1. Hypertensive rats with excessive rising of liver-yang model rats were prepared by spontaneous hypertensive rats (SHR) with drenching Aconiti Praeparatae Decoction.2. Hypothalamic and adrenergdrenic proteins was separated by 2D gel electrophoresis (2-DE). After Coomasie briliant blue G250 staining, gel-image analysis was performed by using PDQuest, we get the differential expressed proteins. 3. The differential expressed proteins was identified by matrix_associated laser desorption/ionization time of flight massspectrometry (MALDI-TOF-MS) and database analysis.RESULTS1. The model of hyperensive rats with hyperactivity of liver-yangwere reproduced succeedly, and the curative effect of Pinggan-Qianyang treatment was good.2. The 2-DE Patterns of hypothalamuses and adrenal glands of normal rats,SHR with hyperactivity of liver-yang and the rats with hyperactivity of liver-yang with Pinggan-Qianyang treatment were presented.Gel-image analysis reveals that there are 27 differential protein spots.3. The 22 of total 25 differential protein spots are successfully identified by MALDI-TOF-MS.The functions of these Proteins involve in metabolism, energy generation, transportation, antioxidation, sigal transduction and cytoskeleton etc according to information provided in NCBI and MSDB database. Among them, there are some proteins closely related to hypertension with hyperactivity of liver-yang and the Pinggan-Qianyang treatment, such as Peroxirdoxin-II,Heat shockprotein-27,Annexin-A1.CONCLUSIONThe blood pressure of hyperensive rats with hyperactivity ofliver-yang is reduced with symptoms ameliorated after taking Pinggan-Qianyang treatment, which might be related to the changes of hypothalamic and adrenergdrenic protein. Appreciated hypothalamic and adrenergdrenic differential expressed proteins with Pinggan-Qianyang treatment succeedly. This approachmay lay a foundation for the further investigation of pathogenic mechanisms in hypertension with hyperactivity of liver-yang and curative effect mechanisms of Pinggan-Qianyang treatment.