Dynamic Study On Serum Pharmacochemistry Of Yin-Huang Oral Liquid And Its Quality Assessment

Objective:An HPLC method was developed to investigate the dynamic fingerprints of serum pharmacochemistry of Yin-huang oral liquid.The HPLC-DAD-ELSD double-fingerprints were used to evaluate the quality of Yin-huang preparation effectively,and the hierarchical cluster analysis and principal component analysis were applied for the quality control of traditional Chinese medicine.Methods:(1)Based on the established HPLC fingerprints of Yin-huang oral liquid,analysis and comparison were carried out among the HPLC profiles of rat serum samples obtained in different time after oral administration of Yin-huang oral liquid.The compounds absorbed into serum,the original crude drugs and their metabolites were identified.(2)Fingerprints of 17 Yin-huang preparations samples from various manufacturers were obtained by HPLC-DAD-ELSD method.The technique of projection discriminate analysis based on hierarchical cluster analysis and principal component analysis was applied to investigate the experimental data.Results:(1)Eleven compounds were detected gradually in rat plasma from 0～180 min after oral administration,seven of which are original form of the compounds in Yin-huang oral liquid and four are metabolites,and the content of them were different in different plasma samples.(2)The fingerprints developed showed that there were differences among the Yin-huang preparation by different manufacturers.HPLC-DAD-ELSD fingerprints and the technique of projection discriminate analysis based on hierarchical cluster analysis and principal component analysis properly revealed the quality information apparently and accurately. Conclusions:(1)The compounds absorbed into blood and its metabolites are the effective constituents and the original crude drugs are the main ingredient drugs of the preparation.The further investigation of serum pharmacochemistry can illuminate the mechanism and effect substances of Yin-huang oral liquid.(2)This method using the hierarchical cluster analysis and principal component analysis can be effectively applied to control the quality of the traditional Chinese medicine.New ideas:(1)The study on serum pharrnacochemistry of Yin-huang oral liquid in different times was investigated for the first time.(2)The HPLC-DAD-ELSD double-fingerprints of Yin-huang preparations were developed for the first time,and the technique of projection discriminate analysis based on hierarchical cluster analysis and principal component analysis was used to quality assessment of Yin-huang preparations for the first time.