Protective Effects Of Atorvastatin Pretreatment Against IARF In Rats

Background and Objective:Renal ischaemia-reperfusion injury is inevitable pathophysiological process in acute kidney injury.There are still existing in acute arteries symptom complex,cardiorenal syndrome,eardiopulmonary resuscitation,cardiopulmonary bypass and operation with renal artery occlusion.Although much attention has been focused on events occurring during the ischemic event or in the early recovery phase,the long-term effects of ischemic injury have received little study.Recently,some studies show that ischaemia-reperfusion injury not only cause acute injury but also lead to long-term renal function deterioration.We explore the transform of ischaemia-reperfusion injury in rat and the effect of medicine intervention study on it.atorvastatin has been shown the protective effect on IRI in different organs,including cardiac muscle,cerebrum, kidney.UP to now,the exact protective mechanism of atorvastatin in the IRI has still not been elucidated.It had been reported that atorvastatin provide protective effect against kidney by nor-lipoidso So it is important to understand the complex pathogenesis of renal IRI and try to find an effective drug to prevent itoTo set up kidney IRI pattern and a week pretreatment on renal recovery in the model of warm ischemia-reperfusion in rats.We tudied and researched the precise mechanism of the disease and the impact ofatorvastatin to the expressed lipid peroxidation(LPO) in ischemic acute renal failure(iARF) rats,to probe mechanism of atorvastatin not comply nor-lipoids in iARF rats treatment.Methods:32 wistar rats were randomly divided into fours:sham operated group and ischemiareperfusion injury(IR group received0.9%saline 2ml/d qd ig.For7 days,atorvastatin control group and atorvastatin treatment group received atorvastatin 20mg/kg.d qd ig.For 7 days,to set up iARF rat model,to put to rats death in batch after 24h,nephrotoxicity was assessed by measuring BUN,Scr,TC,TG, malondialdehyde(MDA) content and supeioxided dismutase(SOD) activity were detected in kidney through kit o Tubular cell apoptosis was observed through optical microscope. Results:IR group induced a significant elevation in BUN,Scr,MDA content as well as severe morphology changes while decreased the renal SOD activity (P＜0.05):Through atorvastatin pretreatment,A+IR group VS IR group,BUN, Scr,obviously was improved(P＜0.05);the express of the nephridial tissue and SOD,MDA obviously was improved too(P＜0.05).However,atorvastatin improved above status of nephrotoxicity.blood-fat of every interclass had no obviously difference((P＞0.05).Conclusions:LPO is one of the important causes of iARF,pretreatment with atorvastatin can protect the rats from ischemia reperfusion injury -induced nephrotoxicity by reducing ROS,While not rely to lower lipoids.