| Objective: To observe the expression of ERαand methylation of its promoter region in bladder cancer cell line T24, and to study whether the lower expression of ERαwas caused by the hypermethylation of its promoter region.Methods: T24 bladder cancer cell lines, in vitro.To use 0.25,0. 50,0. 75μmol/L 5-Aza-CdR treat T24 cells, and 5-Aza-CdR treated group for the experimental group, the control group of untreated cells. And use methylation-specific PCR and fluorescence quantitative pcr observe the methylation status of ERαgene promoter region in experimental and control group cells.The cells of experimental and control groups were injected into mice and gived rised into tumors,using immunohistochemical techniques to observe the ERαexpression in experimental and control groups. The chi-square analysis was used to test difference of ERαexpression in experimental and control groups.Results:1.The ERαgene promoter region of control group cells have a wide range of methylation, modified with sulfite,the promoter region of cytosine remain unchanged, and the other cytosine into thymidine. the status of methylation of ERαgene promoter region decreased significantly in experimental group, all cytosine into thymine, and related with 5-Aza-CdR concentrations. There is low expression of ERαin control group, the expression of experimental group is higher than control group, had statistic significance, P<0.05. 2.The ERαgene promoter region of control group cells have a wide range of methylation, with low expression of ERα.5-Aza-CdR can effectively remove the state of hypermethylation of ERαgene promoter region, and ERαexpression was significantly enhanced.Conclusion: 5-Aza-CdR can effectively removed hypermethylation of ERαgene promoter in bladder cancer cell line T24, and enhanced ERαexpression, inhibited proliferation and reduced invasive ability of T24。And we expected to provide a theoretical basis for 5 - Aza-CdR applied to treat the ERα(-) bladder cancer in furture。... |
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