Study On Tartary Buckwheat Flavonoids And Its Antidiabetes Activity

Buckwheat is a dicotyledon, which grows in cold climate and has short-quarter growth in China. It is a miscellaneous grain crops, which can be also used in pharmacy, and its main active components are rutin, quercetin and D-chiro-inositol etc.In this study, the extract technology of active components in buckwheat bran was established, and the contents of rutin was determined; we studied the regularity of the transformation of rutin to isoquercetin by thermo-chemistry cleavage, then the antidiabetes activity of isoquercetin was studied.An orthogonal design method was used to optimize the extract technology of the active components in buckwheat bran. The factors were extraction method, solvent concentration, ration of solvent: solid, extraction duration and times. And the optimized technology was as following: 60% ethanol of solvent, ten times of ethanol volume/materials weight, one hour of extracting duration and two extraction times, using heating reflux method. The content of rutin in buckwheat with a total number of 166 was determined, and the range extent was 1.4948~1.6244%.A high temperature and pressure method was used to study the transformation of rutin to isoquercetin, using thermo-chemistry cleavage principle. It suggested that the proper condition was that 0.14~0.20 MPa, and the highest yield of isoquercetin was 8.79%.Isoquercetin had an inhibition effect ofα-glucosidase in vitro, and IC50 was 0.75μg/mL.The high dose and middle dose groups of isoquercetin could reduce the blood glucose level significantly in KK-Ay mice, and the oral glucose tolerance test was improved, comparing with the model group. And it could also improve the lipid metabolism, elevate renal function and inhibit pancreatic changes.