Insulin Resistance In Obese Fatty Liver Morbidity Function

Objective:To study the effects of rosiglitazone maleate on nonalcoholic fatty liver disease rats which have insulin resistance and to investigate the roles of insulin resistance and lipids metabolic disorder as well as the function of rosiglitazone maleate in the pathogenesis of nonalcoholic fatty liver disease.Method:The experimental animals(Spraque-Dawley rats)were randomly divided into normal control group(N)and model group(M).The model group was fed with hypsi-fat and hypsi-cholesterol forage for 8 weeks to induce nonalcoholic fatty liver disease(caused by obesity)model which have insulin resistance;and the control group with normal forage.The fatty liver model was established successfully,and was divided randomly into two equal groups:the model control group(D)and the model therapeutic group(S).Physiological saline and rosiglitazone maleate were given to model control group and model therapeutic group respectively for 4 weeks.Then,observing the variation of gross form and histology of liver,and detecting the fasting plasma glucose,the fasting insulin, plasma ApoCâ…¡and ApoCâ…¢by Enzyme-Linked Immunosorbent-Assay; calculating the insulin resistance index by Homeostatic model assessment Insulin resistance index,detecting the enzyme activity of lipoprotein lipase and hepatic lipase,and the express of ApoB-100 mRNA by reverse transcription Polymerase Chain Reaction.Results:1.Compared with group N,the body mass,fasting plasma glucose,fasting insulin and insulin resistance index of group M were higher.And the gross form and histology of liver of group M met the diagnostic standard of fatty liver.The obese fatty liver animal model with insulin resistance were established successfully.2.In comparison with group N respectively,the gross form and histology of liver of group D and S met the diagnostic standard of fatty liver;the fasting plasma glucose,fasting insulin,insulin resistance index and plasma ApoCâ…¢were higher;the contrary to plasma ApoCâ…¡,plasma enzyme activity of lipoprotein lipase,hepatic lipase and the express of ApoB-100 mRNA of liver.Compared with group D,the gross form and histology of liver of group S was better.The fasting plasma glucose,fasting insulin,insulin resistance figure and ApoCâ…¢of the group S was lower;the contrary to plasma ApoCâ…¡,plasma enzyme activity of lipoprotein lipase,hepatic lipase,and the express of ApoB-100 mRNA of liver after 4 weeks' therapy.Conclusions:1.The obese fatty liver disease Spraque-Dawley rats model with insulin resistance can be successfully established by hypsi-fat and hypsi-cholesterol forage.2.Insulin resistance and lipids metabolic disorder played an important role in the morbidity of fatty liver.Rosiglitazone maleate can amendment the insulin resistance of fatty liver rats by lowering the fasting plasma glucose and fasting insulin,improve the enzyme activity of lipoprotein lipase and hepatic lipase by altering content of plasma ApoCâ…¡and ApoCâ…¢,promote the degradation of peripheral very low density lipoprotein and triglycerides,precipitate the express of ApoB-100mRNA in rats' livers,quicken the synthesis of very low density lipoprotein in livers,convey of endogenous triglycerides,and lessen the fatty infiltration of hepatic cells.