The Expression Of ER And ICAM-1 In The Hypothalamus, Pituitary, Ovary And Uterus Of Early Pregnant And Abortive Rats

To investigate the reproductive and physiological effect of ER and ICAM-1and the mediation mechanism of the hypothalamus-pituitary-ovary axis, immunohistochemical ultra sensitive SP method has been used to examine the distribution of estogen receptor(ER) and intracelluar adhension molecule-1(ICAM-1) in the hypothalamus, pituitary, ovary and uterus of the early pregant and abortive rats in the present study. The results are as follows:1. The ER and ICAM-1 positive productions were wildly present in the 21 nuclei of the rat hypothalamus. And were most strongly expressed in the pre-opticus suprachiasmatic nucleus, supraoptic nucleus, suprachiasmatic nucleus, paraventricular nucleus, arcuate nucleus, nucleus anterolateral mamillaris and nucleus mamillaris lateralis, whereas relatively less strongly in the other nuclei. The expression of ER and ICAM-1 in the hypothalamus of the early pregnant rats showed a certain regularity: the variations of ER expression in the preoptic region, supraoptic region, tuberal region and mamillary region show a pretty consistency: ER expression increased durative in the D4, D6 and D9 of pregnancy; Compared with the estrus rats, ICAM-1 expression of the pregnant D4 rats didn't change in the preoptic region, but was reduced significantly in the other regions of the hypothalamus. ICAM-1 expression reached the highest level in each region in the D6 of pregnancy. However, it was reduced dramaticaly, especially in the mamillary region, in the D9 rats, though it was higher in each region than that in the estrus group. Compared with the normal D9 pregnant rats, ER expression in the abortive rats declined in the preoptic region and mamillary region, rose in the supraoptic region, and didn't show obvious change in the tuberal region; ICAM-1 decreased significantly in each region of the abortive rats.2. ER was obviously and clearly present in the pars distalis hypophyseos, pars nervosa hypophyseos and pars intermedia hypophyseos of each group of rats, and was most strongly expressed in the pars intermedia hypophyseos. ICAM-1 positive staining was obvious in each part of the pituitary of the rats, but showed a vague cell outline. The expression of ER and ICAM-1 in the pituitary of the early pregnant and abortive rats showed some certain regularity: ER expression declined in the D4 of pregnancy, increased in the D6 of pregnancy, and increased even higher in the pars distalis hypophyseos and pars nervosa hypophyseos, but not in the pars intermedia hypophyseos, in the D9; ICAM-1 expression in the D4 of pregnancy was lower than that in the estrus rats, but higher in the D6, and reached the peak in the D9. Compared with the normal D9 pregnant rats, the abortive rats showed significantly decline of ER expression in the pars distalis hypophyseos and pars nervosa hypophyseos but pars intermedia hypophyseos; The abortive rats also displayed an obvious drop, especially in the pars intermedia hypophyseos, of the ICAM-1 expression.3. ER and ICAM-1 were both strongly expressed in the germinal epithelium, follicles of different stage, inersticial gland, intersticial cell, vascular endothelium and corpus luteum. But the ICAM-1 positive cells'outline was not as clear as that of the ER positive ones. ER expression showed distinct characteristics in different parts of the ovary of the normal pregnant rats. The estrus rats showed highest ER expression in the primary follicle and secondary follicle, the pregnant D6 rats highest in the corpus luteum and the D9 ones strongest in the inersticial cells and vascular endothelial cells; The changes of ICAM-1 positive staining in each part of the normal pregnant rat ovary put up a fairly consistancy: pregant D4 rats was lower than the estrus ones, hoisted in the D6, and declined in the D9. Compared with the normal pregnant rats, the abortive ones showed significantly decrease of the ER positive production in each part of the ovary, especially in the corpus luteum and intersticial cells; ICAM-1 positive production was detected to be lower in the corpus luteun, but higher in the other parts of the ovary.4. ER and ICAM-1 were both obviously expressed in the stroma, myometrium, decidual and luminal epithelium of the early pregnant rats. And decidua was the main place. There was a fairly regularity of ER and ICAM-1 expression in the early pregant rats: ER expression was higher in the D4 pregnancy than that in the estrus rats, dramatically lower in the D6, and highest in the D9; ICAM-1 increased in the uteri of D4 pregnant rats, peaked in the D6, and declined significantly in the D9. Compared with the normal pregnant D9 rats, the abortive ones showed distinct decrease of ER and ICAM-1 expression in the decidua.