The Experimental Research For Pathologic Change Of Nasal Mucosa In Allergic Rhinitis

[Objective] To establish the model of allergic rhinitis(AR) of Sprague-Dawley(SD) rats and observe the damage of nasal mucosa in morphology and the characteric of pathologic change in nasal mucosa of AR .[Methods] Total 180 healthy SD rats irrespective of female and male were randomly devided into the normal and AR groups it including intermittent allergic group and persistence group respectively . After establishing the model of allergic rhinitis by intraperitoneal injecting comphensive solution of ovalbumin(OVA)and AL(OH)3 then drope 5%OVA solution to nose. Sacrifice rats after 1,2,4,8,12,16weeks ,Apply hematoxylin- ereuth dyeing,make microvascular corrosion casts,transmission electrical microscope to analysis ultrastructure and apply immunohistochemistry technique(SABC method),reverse transcriptio polymerase chain reaction technique to study the protein expressiong of HO-1,TGF-β1,FGF-2 in nasal mucosa AR.[Results] Compare to normal rats,intermittence group: 1)early 4 weeks after intensively sensitizating characteric of morph: endothelial cell edemata,desquamate,textus edemata in lamina epithelialis, goblet cells metaplasia,hypertrophy,productive secretion, angiectasis greatly in propria lamina, glandular organ hypertrophy, collagen oedemata in basal membrane, mucous layer thickening, chaotic textus strcture. Pseudostraified columnar ciliated epithelium or basal layer, with cilia loss and increasing inflammatory cell. eosinophils infiltrating and the expression of HO-1,TGF-β1,FGF-2 are difference significantly. 2) late8,12,16weeks:tissue edema significantly improved, mucosal cells tend to tidy and neatly arranged cilia, the hypertrophic goblet cells decreased significantly, the glands and expansive glandular tube tend to normal. infiltration of inflammatory cells in inherent layer reducing but not completely eliminated, fiber components increased at the same time. eosinophils infiltrating and the expression of HO-1,TGF-β1, FGF-2 are gradually decreasing. Persistence group:1)early 4 weeks after intensively sensitizating characteric of morph : as well as intermittent group, mucosal injuried is very severe. but eosinophils infiltrating and the expres -sion of HO-1 are also not difference significantly compare to intermittence group(P>0.05)。2) late8,12,16 week:damaged epithelium repaired in a certain extent with fibration, but the restoration result of epithelial are untidiness, goblet cell volume is increased submucosal blood vessels increased, vascular wall thickening.especially in the mucous layer a large number of collagen deposited in basement membrane, gland volume increased,hyperplasia,glandular tube structure are fuzzy. further,EOS infiltrating and the expression of HO-1mRNA,TGF-β1,FGF-2 protein were significantly different compared to intermittence group(P <0.05). In the progress of remodeling the symptom of AR rats have the trend to reduce.[Conclusion] two groups of AR mucosa before 4 weeks there are mucosa damage,while after 4 weeks they are very different in pathologic change. compare to the intermittence group,the persistence group mucosa are remodeling to repair the damage of mucosa,it maybe the response of damage. Pathologiccharacteristic:repair after cell loss, subepithelial fibrosis, goblet cell proliferation, vascular proliferation, irregular thickening of the lumen, the gland volume increased, collagen deposition increasing in the basement membrane layer. While intermittence group rat mucosa remodeling aren't significant, it maybe the recovery become more easy after deprivating allergen. In the progress of remodeling the nasal mucosa inflammation and oxidative damage have the trend to reduce .and TGF-β1,FGF-2 are participate in fibrosis repairing.