The Epidemiological Survey Of The Active Infection Of Human Cytomegalovirus In Several Territories Of Hebei Province

Objective:Increasing attention is being paid to the viral etiology of atherosclerosis(AS)for more and more scholars Since Fabricant inoculated Marekvirus to turkey and made it provoke atherosclerosis,and Human cytomegalovirus(HCMV) is thought to be the most possible etiological factor of atherosclerosis.We have proved that the active infection of HCMV was one of the risk factors of atherosclerosis in our previous study.Domestic and foreign scholars have been studying the topic in different aspects.There are many reports about the relationship between HCMV and atherosclerosis,but the findings are still in controversy.The surrounding of laboratory can not reflect the actual conditions exposed externally,while epidemiology avoids the shortcoming by studying the men exposed physical environment.The combination between laboratorial study and epidemiology will proof the topic in different directions.The present epidemiological datas are concentrated mostly in pregnant women,newborn infants,blood donors,organ transplanting receptors,immunologic deficitors(as multiple sclerosis,AIDS and so on),which can not reflect the infecting state in the whole crowds.HCMV-pp65 is a favourable late antigen which can express in peripheral blood monouclear cells,nertrophile polymorphonuclear leucocytes,vascular endothelial cells when HCMV is in reactiveness while can not be detected when in latent state.Detecting HCMV-pp65 has been one of the achnowledged internationally standard methods of diagnosing HCMV infection in reactiveness.We will carry out epidemiological survey about HCMV-pp65 in 11 administrative areas of Hebei Province,return to visit the surveying crowds,carry out prospective study,observe the occurrence of diseases related with atherosclerosis especially cerebral infarction.We will investigate the relationship between HCMV and atherosclerosis on epidemiology,research furtherly the etiological factors and risk factors of atherosclerosis so that we can go in search of the novel mode of preventing and curing ischemic cerebrovascular disease, which is the major purpose of the epidemiological survey.Method:The immunohistochemical method was applied to detect cytomegaloviral late-antigen pp65 in leucocyte of peripheral blood.The article analyzed the epidemiological survey of the positive rate of HCMV-pp65 in crowds in several territories of Hebei province(Shijiazhuang,Gaocheng, Liuxinzhuang,Zhaoxian,Gaoyang,Lixian,Fuping,Xianxian, Zanhuang,Nanpi,Shexian,Wuyi,Chongli).The total number of surveying group was 1954 consisted of 781 men and 1173 women,the mean age was(55.16±10.03)years old.All of the surveying counties or cities were divided to 6 administrative areas:Shijiazhuang territory,Baoding Territory,Cangzhou territory,Handan territory,Zhangjiakou territory and Hengshui territory.Make data pool by microsoft excel,import the data pool into SAS data set,analyze the positive rate of HCMV-pp65 about territory,age,sex and so on by SAS 6.12 statistical software.Numeral data by x~2 test or corrected x~2 test,α=0.05.Results:1 The immunohistochemical method was applied to detect cytomegaloviral late-antigen pp65 in leucocyte of peripheral blood from 1954 samples.Negative cells were in uniform violet while the kytoplasm and(or)nucleus of positive cells were in brown.The positive conclusion should be made if positive cells exceeded 1/50000.2 The immunohistochemical method was applied to detect HCMV-pp65 in leucocyte of peripheral blood from 1954 samples included 1807 from countryside and 147 from town, the mean age was(55.16±10.03)years old.The total positive rate of HCMV-pp65 was 17.14%in global crowds.There was no statistical difference about the positive rate of HCMV-pp65 on sexes in global crowds,x~2=0.283,P＞0.05;There was statistical difference about the positive rate of HCMV-pp65 on ages in global crowds,x~2=8.688,P＜0.05,60-stage was the highest(20.74%)and 40-stage was the lowest(14.11%);There was statistical difference about the positive rate of HCMV-pp65 on territories in global crowds,x~2=55.246,P＜ 0.05,Baoding territory ranked highest(26.65%),followed by Cangzhou territory(23.32%),Handan territory(22.22%), Zhangjiakou(19.57%),Hengshui(11.95%)and Shijiazhuang (11.49%).3 The positive rate of HCMV-pp65 was 18.04%in rural crowds included 1807 samples,the mean age was (55.87±10.19)years old.There was no statistical difference about the positive rate of HCMV-pp65 on sexes in rural crowds, x~2=1.948,P＞0.05.There was no statistical difference about the positive rate of HCMV-pp65 on ages in rural crowds,x~2= 6.824,P＞0.05.There was statistical difference about the positive rate of HCMV-pp65 on territories in rural crowds,x~2= 44.386,P＜0.05,Baoding territory ranked highest(26.65%), followed by Cangzhou territory(23.32%),Handan territory (22.22%),Zhangjiakou(19.57%),Shijiazhuang(12.52%)and Hengshui(11.95%).4 The positive rate of HCMV-pp65 was 6.12%in urban crowds included 147 samples,the mean age was(46.87±7.54) years old.There was no statistical difference about the positive rate of HCMV-pp65 on sexes in urban crowds,x~2=0.035,P＞0.05.There was no statistical difference about the positive rate of HCMV-pp65 on ages in urban crowds,x~2=1.624,P＞0.05.5 There was statistical difference about the positive rate of HCMV-pp65 between rural crowds and urban crowds,x~2= 13.594,P＜0.05.6 There was no statistical difference about the positive rate of HCMV-pp65 in crowds between plain area and mountain area,x~2=2.396,P＞0.05.Conclusions:1 The positive rate of HCMV-pp65 was 17.14%in several territory of Hebei Provence which indicated there was activated infection of HCMV in common crowds;2 There was statistical difference about the positive rate of HCMV-pp65 among territories;3 There was no statistical difference about the positive rate of HCMV-pp65 on sexes but the positive rate of HCMV-pp65 was higher slightly in male than female;4 There was statistical difference about the positive rate of HCMV-pp65 on ages in global crowds while there was no statistical difference on ages in rural crowds and urban crowds but a rising trend with growing ages;5 There was statistical difference about the positive rate of HCMV-pp65 between rural crowds and urban crowds then it was higher obviously in rural crowds than in urban crowds;6 There was no statistical difference about the positive rate of HCMV-pp65 in crowds between plain area and mountain area.7 It could be inferred the active rate of HCMV in immunocompetent crowds should be lower than 17.14%.