The Biological Significance And The Related Study Of Androgen Receptor, Ki67 And Basic Fibroblast Growth Factor In Benign Prostate Hyperplasia, Prostatic Intraepithelial Neoplasms And Prostate Cancer

Objective: We intended to detect the expressions of Androgen Receptor (AR), Ki67 and basic Fibroblast Growth Factor (bFGF) in prostate cancer (Pca), prostatic intraepithelial neoplasms (PIN) and benign prostate hyperplasia (BPH) and to discuss their relationship with clinicopathological features in Prostate cancer. Moreover, we analyze the probably related mechanism among three protein in Prostate cancer. With these results, We hoped to be helpful for early diagnosis, gene therapy and prognosis evaluation of Prostate cancer.Material and Method: All samples in our research were collected from two general hospitals in Tianjin from 2004 to 2006. All patients were not enforced radiotherapy, chemotherapy and hormone therapy before operation and were diagnosed by Haematoxylin—eosin dyeing of histopathology. All samples were collected from patients of transurethral resection or radical excision, in which there were 40 cases of prostate cancer, 20 cases of prostatic intraepithelial neoplasms and 20 cases of benign prostate hyperplasia. We used SP immunohistochemistry method to detect the expressions of AR, Ki67 and bFGF proteins. All data were processed by SPSS version 11.5 analysis software.Result:1. Androgen Receptor protein were respectively detected in 60.0%(24/40), 75.0%(15/20)and 90.0%(18/20)of Prostate cancer, prostatic intraepithelial neoplasms and Benign prostate hyperplasia. The positive rate is significantly difference in total (p<0.05), AR protein in Benign prostate hyperplasia is significantly higher than that of Prostate cancer (p<0.05). The positive rate of AR protein in PIN compared with Pca or BPH is no significant difference (p>0.05).2. The positive rate of AR protein in 40 cases of Pca with different Gleason grade respectively are :well differentiated group 100.0%(11/11), middling differentiated group 75.0%(9/12), poorly differentiated group 16.7%(4/17), the positive rate is significantly difference in total (p<0.01). AR protein in well differentiated group is significantly higher than that of poorly differentiated group (p<0.01), and the positive rate of AR protein in middling differentiated group is significantly higher in poorly differentiated group (p<0.01). There are no significant difference between well differentiated group and middling differentiated group in Pca (p>0.05).3. The positive rate of AR protein was significantly higher in the group without metastasis than that with metastasis in Pca (p<0.01). 4. In Pca, the expression degrees of AR protein has significantly negative correlation with the differentiation degrees (p<0.05), and significantly positive correlation in different clinic stage (p<0.05).5. Ki67 is respectively detected in 67.5%(27/40), 50.0%(10/20)and 15.0%(3/20)of Prostate cancer, Prostatic intraepithelial neoplasms and Benign prostate hyperplasia. The positive rate in Pca is significantly higher than that in the group merging PIN and BPH (p<0.05).6. The positive rate of Ki67 protein in 40 cases of Pca with different Gleason grade respectively are :well differentiated group 45.4%(5/11), middling differentiated group 75.0%(9/12), poorly differentiated group 76.5%(13/17), the positive rate is significantly difference in population (p<0.05). Ki67 protein in poorly differentiated group is significantly higher than that of well differentiated group (p<0.05), There are no significant difference between middling differentiated group and poorly differentiated group or middling differentiated group and well differentiated group in Pca (p>0.05).7. The PSA level in the positive group of Ki67 is significantly higher that in the negative group (p<0.01).8. Ki67 is respectively detected in 100.0% and 56.7% of metastasis and without metastasis groups in Pca, and the positive rate of Ki67 in metastasis group is more significantly higher than that of without metastasis group in Pca (p<0.05).9. In Pca, the expression degrees of Ki67 protein has significantly positive correlation with the Gleason grade (p<0.05), but it is no significantly correlation with different clinic stage (p>0.05).10. bFGF protein are respectively detected in 57.5%(23/40), 30.0%(6/20) and 15.0%(3/20) of Prostate cancer, Prostatic intraepithelial neoplasms and Benign prostate hyperplasia. The positive rate is significantly difference in population (p<0.01), and bFGF protein in Prostate cancer is significantly higher than that of Benign prostate hyperplasia (p<0.01). bFGF protein in Prostate cancer is significantly higher than that of PIN (p<0.05), But the positive rate of bFGF protein is no significant difference between PIN and BPH (p>0.05).11. The positive rate of bFGF protein in 40 cases of Pca with different Gleason grade respectively are :well differentiated group 45.4%(5/11), middling differentiated group 50.0%(6/12), poorly differentiated group 70.6%(12/17), the positive rate is no significantly difference in population (p>0.05). The positive rate of bFGF in metastasis group is more significantly higher than that of without metastasis group in Pca (p<0.01).12. The expression degrees of bFGF protein have no correlation with the Gleason grade in Pca (p>0.05), and have positive correlation with clinic stage (p<0.05) .13. In Pca, the expression degrees of AR protein have significantly positive correlation with the bFGF expressing degree (p<0.05), but there is negative significant correlation between AR and Ki67(p<0.05). Conclusions:1. The positive rate of AR protein is gradually higher in order Prostate cancer, Prostatic intraepithelial neoplasms and Benign prostate hyperplasia, which suggest that AR expressing may be related to the genesis and progress of Prostate cancer, it may play an important role in the early stage of Prostate cancer; AR may have important value in early stage diagnosis of Prostate cancer, Prostatic intraepithelial neoplasms and Benign prostate hyperplasia.2. The positive rate of AR protein is higher in metastasis group than that of metastasis group in Pca, which suggest that AR expressing may be related to infiltration and metastasis of Prostate cancer, and be related to the progression and malignant biological behavior;It can be a reference factor in prognosis evaluation.3. The positive rate of AR protein in well differentiated group is significantly higher that of poorly differentiated group in Pca, which suggest AR may be played an important role on inhibiting infiltration and metastasis and may promote differentiated of cell. It can be a important reference factor in prognosis evaluation.4. The positive rate of Ki67 in PCa is significantly higher that in merging groups of Prostatic intraepithelial neoplasms and Benign prostate hyperplasia, the positive rate of Ki67 in poorly differentiated group is significantly higher that of well differentiated group in Pca and the positive rate of Ki67 in metastasis group is significantly higher that of without metastasis group in Pca ,which suggest that Ki67 may play a important role in the genesis and development of Prostate cancer. It is not only promote cell mitosis and cell proliferation activity, but also reflect differentiation potency and evaluate the potency of infiltration and metastasis in cancer cell. It may suggest that Ki67 could be a good marker to identify whether prostate disease is innocence or malignant and help to estimate genesis of prostate cancer and estimate therapeutic efficacy.The positive rate of bFGF protein in prostate cancer is higher that of Prostatic intraepithelial neoplasms and Benign prostate hyperplasia and in metastasis group it is higher that of without metastasis group in Pca ,which suggest that bFGF play a important role in the genesis and development of Prostate cancer.6. In PCa, the expression degrees of bFGF protein have significantly positive correlation with the clinic stage. Which suggest that bFGF can play along with infiltration and metastasis of tumor through promoting tumor proliferation and angiogenesis ; It can be a reference marker of prognosis evaluation.7. AR, Ki67 and bFGF play an important role in the genesis and development of Prostate cancer, the combination of effect contribute to genesis, development and metastasis in tumor cells. combination detection among AR, Ki67 and bFGF may be the specific marker in early diagnosis of Pca.