The Application Of P16 And Ki67 Detection In The Diagnosis Of Cervical Intraepithelial Neoplasia

Objective:To observe the expressions of P16 and Ki67 in cells of cervical intraepithelial neoplasia(CIN) and to analyze the relationships between the groups with clinical pathology. Methods:92 cases of exfoliated cells of patients with CIN who treated with resection in Changchungynecology and obstetrics hospital from January 2012 to December 2015 were selected as the observation group. The 92 cases in the observation group were divided into 3 branch groups by grade CIN I with 30 cases(observation A group), grade CIN II with 34 cases(observation B group) and grade CIN III with 28 cases(observation C group). Another 35 cases of exfoliated cells of patients with non-cervical lesion treated with resection in the same hospital during the same period were selected as the control group. The expressions of multiple tumor suppressorP16(P16) and proliferating cell nuclear antigen Ki67(Ki67) in different exfoliated cells were detected by immunocytochemical method. The results of the 3 branch groups in the observation group were compared between which the relationships with clinical pathology were discussed. Results:(1) The expressions of P16 and Ki67 have statistical significance between the observation group and the control group(P<0.05). The positive rates of P16 and Ki67 in the observation group were respectively 88.04% and 85.87%, which in the control group were respectively 54.29% and 51.43%. The former were significantly higher than the latter(P<0.05).(2) There were statistical significance in the level differences of the expressions of P16 and Ki67 among the observation A group, observation B group and observation C group(P<0.05) and the positive rates of P16 and Ki67 in the three groups were respectively 76.67%, 73.33%, 88.24% and 85.29%,100.00%, 100.00%. There were significant differences between each two groups(P<0.05).(3) The more serious the CIN conditions, the higher the P16 and Ki67 positive expression rates. It showed that the expressions of the two were positively associated with clinical grading(r=0.453,0.512).(4) By P16/Ki67 double staining in the different grades of CIN samples of exfoliated cells, we observed there are no P16/Ki67 double staining positive cells in group A; five samples were exist P16/Ki67 double staining in group B; in group C, p16 and Ki67 positive cells were observed rate was 100%. Conclusion:(1) In patients with CIN cervical exfoliated cells, P16 and Ki67 positive expression rate was significantly higher than that in normal healthy people and with the severity, the positive rate significantly increased.(2) P16 and Ki67 positive expression rate and CIN clinical classification has positive correlation. So P16 and Ki67 can be used as an important indicator of CIN disease severity and deterioration of potential assessment in clinical diagnosis and treatment.(3) P16/Ki67 double staining provide a reliable theoretical basis for CIN further diagnosis and treatment. It improves the clinical triage management of cervical cancer screening and help to diagnosis and guidance the treatment of the disease.