Study On Benzylisoquinoline Alkaloids Metabonomics In Plant Of Macleaya

Due to benzylisoquinoline alkaloids pharmacological activity, the researchers all over the world have paid much attention to it. All of these alkaloids are biosynthesized in the metabolism network which have the same origin. This paper uses Macleaya cordata (Willd.) R. Br., in which many benzylisoquinoline alkaloids have been isolated, as research object, and a more comprehensive and systematic research for benzylisoquinoline alkaloids metabolism is carried out by integrating high-throughput analysis technology and complex metabolic network research methods. The details are summarized as follows:1. The concept of benzylisoquinoline alkaloids metabonomics was proposed on the basis of plant chemotaxonomy, plant metabonomics and benzylisoquinoline alkaloids secondary metabolism. Structure of the benzylisoquinoline alkaloids metabolic network was studied in detail, and the weighted adjacent matrix was used to describe metabolism network, also its significance in the field of benzylisoquinoline alkaloids resource development, chemotaxonomy and authentic medicinal herbs was discussed.2. A high-performace liquid chromatographic method coupled with UV detector was developed to simultaneously analyze protopine, allocry-ptopine, sanguinarine and chelerythrine in M cordata (Willd.) R. Br., which have transforming relations. The proposed method was applied to determine dynamic changes of the four alkaloids in different parts of M cordata (Willd.) R. Br. during growing period. 3. Given the poor compatibility between the mobile phase of the present system using for alkaloids analyzing, and HPLC/MS, we investigated the effect of perfluorinated carboxylic acid and carboxylic acid additive on the alkaloids separation in different column and mass spectrometry response in detail and a method of HPLC/DAD/ESI/MS was developed which the separation and mass spectrometry response can reach good results at the same time by the rational combination of high carbon content column and mobile phase with carboxylic acid, Combined with the knowledge of benzylisoquinoline alkaloids metabolic pathway, benzylisoquinoline alkaloids in M cordata ( Willd. ) R. Br. were identificated preliminaryly.4. The database which contains molecular weight, mass spectrometry features, UV features, as well as reduction characteristics of alkaloids in biosynthesis pathway, was established for data matching. We analyzed the different parts of M cordata (Willd.) R. Br. and M microcarpa (Maxim.) Fedde using the method proposed the previous chapter, identificated preliminaryly benzylisoquinoline alkaloids by matching the data obtained and compared the differences of benzylisoquinoline alkaloids metabolism between two species by combining with benzylisoquinoline alkaloids metabolic network.