| Objective: To investigate the distribution of single nucleotide polymorphisms(SNPs) on retinol binding protein 4(RBP4) gene and FOXO1 gene, and their relationships with the occurrence of type II diabetes mellitus (T2DM) in Chinese Han population.Methods: Totally ten SNPs on RBP4 and FOXO1 were determined in 384 T2DM patients and 384 normal controls by TaqMan plobe genetyping and agarose gel electrophoresis methods, and their serum level of fasting blood glucose(FBG), total cholesterol(TC) and triglyceride(TG) were also estimated.Results: For RBP4, there was no significance in various genetypes and alleles of -803,G>A;+5169,C>T;+6969,G>C between two groups (p>0.05). every genetype had no relationships with T2DM (using adjusted logistic regression models). No haplotype was associated with T2DM.For FOXO1, Among seven SNPs typed, significant variation was found in the frequency of rs7324943 G/T in the two groups, and further stratification analysis showed that in subjects of aged>40 and non-hypertension, there was a higher risk of T2DM in GT heterozygous carriers than in GG homozygous carriers (OR=1.47,1.80), T allele carriers showed higher risk than non-T carriers (OR=1.42,1.79). For rs17592236 C/T, though no significant frequency variation was found between two groups, but in subjects of aged≤40, stratification analysis showed dramatically increased risk of T2DM in CT and TT carriers than in CC carriers (OR=6.33,10.15), T allele carriers showed 7.11-fold higher risk than non-T carriers. A haplotype CT related to T2DM susceptibility was also found, which could decrease the risk of its carriers by 28%.Conclusion: For RBP4, the polymorphism of -803 G>A,+5169 C>T,+6969 G>C had no relationships with T2DM in Chinese Han population. For FOXO1,the polymorphism of rs7324943 G/T,rs17592236 C/T and a haplotype CT were found related to the susceptibility of T2DM in Chinese Han population. Yet further studies are necessary to explain the impact of these polymorphisms on the disease occurrence. |
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