| Objective: To study the effects of Arnebia euchroma (Royle) Johnston extracts (fat-soluble extract, AE-â… ; water-soluble extract, AE-â…¡) on life-span in drosophila melanogaster (fruit fly) and on anti-aging in subacute aging mice caused by D-galactose, and the effective components initially. Methods: The effect of AE extracts on the mean and maximum life-span of fruit flies were observed, and D-galactose-aging mice model was established by injection of 1.2% D-galactose 10ml/kg. The coefficient of thymus and spleen; blood urea nitrogen (BUN) content and total antioxide capacity (T-AOC) activity in serum; superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) content in liver tissue and monoamine peroxidase (MAO), nitricoxide synthase (NOS) activities in brain homogenate were measured. Results:â‘ The mean of both male and female fruit flies fed on media containing AE-â… all dosage and AE-â…¡middle and high dosage were significantly longer than those fed on pure maize powder media(P<0.05 or P<0.01); AE-â… low and middle dosage groups and AE-â…¡m iddle and high dosage groups could significantly prolong the maximum life-span in the male fruit flies(P<0.05 or P<0.01); AE-â… low and middle dosage groups and AE-â…¡h igh dosage groups could significantly prolong the maximum life-span of the female fruit flies compared with the control group(P<0.05 or P<0.01).â‘¡Compared with the D-galactose-aging model group, AE-â…¡middle and high dosage groups could significantly increase the thymus index (P<0.05 or P<0.01); both AE-â… a nd AE-â…¡all dosage groups could obviously reduce the content of serum BUN in mice (P<0.01); Except AE-â…¡l ow dosage group, AE-â… a nd AE-â…¡all dosage groups could significantly increase the activity of liver homogenate T-SOD(P<0.01); AE-â… middle and high dosage groups could strengthen the GSH-Px activities in liver homogenate (P<0.05 or P<0.01); The liver MDA levels in AE-â… a ll dosage groups and AE-â…¡high dosage group were all obviously decreased, which were significantly higher than that in the control group (P<0.05 or P<0.01); AE-â…¡a ll dosage groups could obviously decrease the activities of MAO and NOS in brain tissue(P<0.01); but AE-â… all dosage groups had no marked effects on the index of brain homogenate mentioned above(P>0.05). Conclusions: AE-â… a nd AE-â…¡p rolong the life-span of fruit flies and showed significant anti-aging effects in subacute aging mice caused by D-galactose. |
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