Dynamic Changes And Correlation Research Of Peripheral Blood CD34~+ Cells In Patient With Craniocerebral Trauma

Objective The study was designed to research the dynamic Changes of peripheral blood CD34⁺ cells(PBCD34⁺)in patient with craniocerebral trauma,and the correlation of which between the pathogenetic condition and Prognosis.Meanwhile to investigate the relationship expression of peripheral blood between PBCD34⁺ cells in the patients with craniocerebral trauma in convalescence stage during hyperbaric oxygen treatment.We also research the role of PBCD34⁺ at repairing in trauma.Methods in phase 1,38 patients with craniocerebral trauma grouped according to the Glasgow coma Scale(GCS)have been selected..20 normal people for contrast.The PB CD34⁺ cells were countted dynamicly with ProCOUNT by flow cytometry and WBC were countted dynamicly by automatic cytoanalyze on the 1^stday.3^rdday,5^thday 7^thday 14^thday.In phase 2,11 patients with craniocerebral trauma in convalescence stage were selected for HBO treatment group,the number of peripheral blood CD34⁺ cells and WBC has been tested before and in each of the first 5 weeks during the treatment of HBO,11 ordinary people for control group.Results In phase 2,the number of PB CD34⁺ cells rise after head injuries, contrast with the control group the high GCS score group shows significance in the 7^th day and 14^thday.the low GCS score group shows significanc in the 3^rdday,5^thday,7^th day 14^thday.and the ascensus of PB CD34⁺ cells in low GCS score group exceed these in high GCS score group.On the 5^thday 7^thday 14^thday showed the significance(P＜0.05). WBC set up immediately after head injuries and to descend gradually which has inverse correlation with the change of PBCD34⁺ cells,in low GCS score group(p＜0.05),the PB CD34⁺ cells in patients who are eusemia(GOS=5)rise earlier and maintain longer time than which in patients who are unfavourable prognosis(GOS＜5).In phase 3,during the HBO treatment the Absolute value of PBCD34⁺ cells shows wave characteristics:the number of PBCD34⁺ cells reduced in the 1^stand the 3^rdweeks,but increased in the 2^nd and the 5^thweeks;the number of PBCD34⁺ cells in the 2^ndand the 5^thweeks had statistical significance compared with that of the control group and before the treatment of HBO(p＜0.05).In the treatment group,the number of CD34⁺ cells in the 1^stand the 3^rd weeks had statistical significance compared to that in the 5^thweek(p＜0.05).After HBO treatment the average number of CD34⁺ cells exceed the number before HBO treatment and control group,which have statistical significance(p＜0.05).Conclutions 1.there is a self-mobilize PBCD34⁺ cells after head injuries,organism may via self-mobilize PBCD34⁺ cells to repair head injurry,2.dynamic monitoring PBCD34⁺ cells and valuable for the judgement of Prognosis and treatment in craniocerebral trauma.3.HBO can mobilize bone marrow derived stem cells marked by CD34⁺ cells,and can also participate in the restoration of craniocerebral trauma in convalescence stage via mobilizing CD34⁺ cells.