Matrix-mediated Retention Of Osteogenic Differenttiation Potential By Rat Bone Marrow Stromal Cells During Ex Vivo Expansion

ObjectiveTo investigate the influence of denatured collagen I on the proliferation and osteogenic differetion potentional of rat bone marrow stem cell ex vivo expansion.MethodsThe cultured rat bone marrow stem cells were separated into 2 groups(A and B) at random. Group A was cultured on denatured collagen I matrix and group B was cultured on tissue cultured plastic. The proliferation capacity and the rate of apoptosis of both late passage cells were detected. And also the ALP was detected by quantitative assay and staining after osteogenic differetion.ResultsWe found that growth of BMSCs on a DC matrix versus tissue culture pla- stic significantly reduced the rate of apoptosis,increased the proliferation capacity of ex vivo expanded BMSCs, and resulted in a dramatic increase in the retention of the potential to express osteogenicspecific functions and markers upon treatment with osteogenic stimulants.ConclusionThe growth of BMSCs cultured on DC matrix can result in the reductionof the rate of the cell aging when cultured in vitro. And also increased the expressionof osteogenicspecific marker after osteogenic stimulant. The experiment found a proach to provide a great quantity cells to tissue engineering.