| Objective Uses the enzyme association immunity adsorption law examination coronary disease group to come of age worry merge diabetes group and the normal control group blood serum matrix metal proteinase– 9,simultaneously examines blood pressure (BP),body weight index (BMI),Empty stomach blood sugar (FBG),Meal latter two hour blood sugar (PBG),The blood fats and so on a series of targets, from in above target dynamic change and the correlation analysis and the multiple regression analysis understood they respective change rule, promulgates MMP-9 at coronary disease, coronary disease merge diabetes occurrence, in the development the institute function, and has provided a bridge for between diabetes and cardiovascular disease relational research, This experimental design will examine:Whether MMPs comes of age worry merge diabetes at coronary disease in the occurrence development to play the very important role; MMP-9 might whether take the judgment gruel type hardening mottling unstable serology target, and will have the good clinical significance in the acute coronary artery syndrome morbidity early time; Whether the blood fats metabolism disorder did participate in two kind of disease occurrence development; Whether two group of disease MMP-9 level and the blood fats level do have the correlational dependence;Causes clinical cardiovascular doctor to take the cardiovascular disease the sugar metabolism unusual condition, improves patient's life style, reduces the cardiovascular disease the formation rate and the mortality rate.Methods Uses the group comparison research. The coronary disease group: Elects from June, 2006 to February, 2007 coronary disease patient who attaches the courtyard heart two branch in hospitals in the Yan'an University medicine, total 69 examples, acute coronary syndrome 42 examples (acute myocardial infarction 22 examples, unstability mode angina pectoris 20 examples) stability mode angina pectoris 27 examples, age between 45-79 year old. The coronary disease merge diabetes group: Elects February, 2007 to attach the courtyard heart two branch in hospitals from June, 2006 to in the Yan'an University medicine coronary disease to merge 2- diabetic, total 22 examples, age between 43-78 year old. Normal control group: Same time attaches the courtyard physical examination center selection age in the Yan'an University medicine, the sex, healthy physical examination 20 which the body weight index matches (male 10, female 10), Age between 42-75 year old. after the examination specimen fasts 12h the trying over night,The early morning gets up empty stomach extraction elbow venous blood 4mL,Conventional separation blood serum,The preservation in– 20℃; treats the determination, MMP-9 (reagent provides with the ELISA law determination blood serum in by Shanghai Wheat Sha Bio-engineering Limited company), the MMP-9 density unit is ng/ml, the sensitivity is 0.156ng/ml, Uses the Austrian napier Kang company (TECAN SUNRISE) the completely automatic enzyme sign meter examination, determines the blood sugar, the blood fats, the survey blood pressure, computation body weight index (BMI) ;The determination step and the judgment standard carry on strictly according to the stipulated procedure, for avoids approving the error and the measuring error, the above examines collects disposable defrosting after the complete specimen, examines in groups, the blood fats, the blood sugar defer to the hospital convention clinical examination method.The main statistical target uses the normality examination, The normal distribution each statistical measurement target uses the mean value±standard deviation to indicate,During not group, in compares uses the single factor variance analysis, the F-test,Around the treatment comparison uses pairs the T-test,The correlation analysis uses the multi-dimensional return law,All data feeds the computer,With SPSS13.0 software processing, uses two-sided P<0.05 is the statistics significance meaning .Between two variable relevant analyses uses the simple correlation analysis. Results three group of crowd common material comparison: Patient's age, sex constitution as well as body weight index comparison difference non-significance significance;three group of crowd's total cholesterol levels respectively be 5.59±0.46mmol/l,6.18±0.73mmol/l,4.82±0.55mmol/l, three group of mean value comparison difference have the significance meaning , three group of mean value 22 comparison differences have the significance meaning (P value <0.001); Three group of crowd's LDL-C levels respectively be 3.49±0.54mmol/l, 4.53±0.75mmol/l, 3.09±2.16mmol/l, three group of mean value comparison difference have the significance meaning, three group of mean value 22 comparison differences have the significance meaning;three group of crowd's MMP-9 densities respectively are 83.21±26.22 ng/ml, 97.24±8.70 ng/ml, 53.88±7.66 ng/ml.Three group of mean value comparison difference has the significance meaning (P value <0.001), Three groups of 22 comparisons, coronary disease group and coronary disease merge diabetes group and the control group comparison difference have the significance meaning (P value <0.001), but coronary disease group and coronary disease merge diabetes group comparison difference non-significance significance (P value =0.868);in coronary disease group according to AMI, UA and SA divides into three subnits, again makes three groups of comparisons, three group of crowd MMP-9 density respectively be 112.63±16.89 ng/ml, 86.61±9.40ng/ml, 56.62±5.50ng/ml. During three groups is the comparison difference to have the significance meaning (P value <0.001); Three group of mean value 22 comparison differences have the significance meaning (P value <0.001 or P value <0.05);presses the stability mode angina pectoris, not the stability mode angina pectoris to the acute cardiac arrest from light to heavy rank arrangement, discovered the MMP-9 density from low to high arranged in order, uses the Spearman rank correlation analysis, prompts the MMP-9 level and coronary disease event serious degree present correlation rs=0.910, P<0.001);coronary disease group patient blood serum MMP-9 level and blood plasma TC, the LDL-C density has the correlational dependence (r=0.401 or r=0.195, P value <0.001 or P value <0.05).Conclusion This research demonstrated MMP-9 comes of age in worry merge diabetic at coronary disease the normal control group obvious markup (P value <0.001), but coronary disease group and coronary disease merge diabetes group comparison difference non-significance significance (P value =0.868); The blood serum MMP-9 level is higher than the stability mode angina pectoris and the healthy control group obviously in the acute coronary artery syndrome group (P value <0.001 or P value <0.05), But stability mode angina pectoris patient's MMP-9 horizontal and the normal crowd compares then not obvious difference; In the subnit analysis, the acute cardiac arrest patient's MMP-9 level is higher than the unstable angina pectoris patient obviously, the latter MMP-9 level is higher than the stability mode angina pectoris patient obviously (P value <0.001 or P value <0.05), According to stability mode angina pectoris, not stability mode angina pectoris, acute cardiac arrest pathological change from light to heavy rank graduation, MMP-9 level change and coronary disease event serious degree present correlation (rs=0.910, P<0.001); Coronary disease and coronary disease merge diabetes group patient TC, LDL-C obviously are higher than the control group, in coronary disease patient MMP-9 and TC, LDL-C present correlation (r=0.401 or r=0.195, P value <0.001 or P value <0.05).
|
PDF Full Text Request