Synergistic Influence Of Curcumin And Cisplatin On Androgen Independent Prostate Cancer Cell Line PC-3

Objective: To study the growth inhibition effects of Curcumin and Cisplatin on androgen independent prostate cancer cell line PC-3.Methods: The growth activities of cancer cells were studied by MTT colorimetry. Cell cycle phases and the apoptosis were inspected by flowcy-tometery (FCM). The morphological changes of cancer cells were observed under electronic microscopy.Results: Curcumin obviously suppressed the proliferation of PC-3 cell line in does-dependent and time-dependent manners in vitro. There was an obvious difference of inhibition rate in different concentrations and time groups (P<0.05). Curcumin could lead to characteristic morphological changes of apoptosis in PC-3 cell line after 24 hours. The ratios of apoptosis cell were significantly higher than that of controls (P<0.05) after curcumin influence the PC-3 cell line. Curcumin could arrest the cell cycle of PC-3 cell line at G2/M phase in does-dependent manners (P<0.01). Thses effects could enhance significantly by cisplatin. The curcumin and cisplatin can all induce cell line PC-3 apoptosis. When the two drugs combined, the inductive effect for cell apoptosis became obvious.Conclusion: Curcumine could suppress the growth of androgen-indenpent prostate cancer cell lines and promote their apoptosis by different mechanism. Cisplatin can enhance antiproliferative effect of curcumin to hunman prostate cancer cell line PC-3.