Curcumin-induced The Apoptosis Of Androgen-dependent And Androgen-independent Prostate Cancer Cell Lines In Vitro

Purpose: To explore the apoptosis of curcumin on androgen-dependent (LNCaP) and androgen-independent (PC3) prostate cancer cell lines in vitro.Methods: After LNCaP and PC3 cell lines were affected by 10, 25, 50, 75, 100 μmol/L curcumine respectively, the cell activity was assayed with MTT method at 5, 12 and 24 hours respectively; Electronic microscopy and flow cytometry were adopted to observe the morphological changes and the cell cycle of LNCaP and PC3 cell lines at 24 hours. After 5 hours, the expressions of IκBa in LNCaP and PC3 cell lines was observed with western blotting.Results: Curcumin obviously suppressed the proliferation of LNCaP and PC3 cell lines in does-dependent and time-dependent manners in vitro. There was an obvious difference of inhibition rate in different concentrations and time groups (P<0.01). Curcumin could lead to characteristic morphological changes of apoptosis in LNCaP and PC3 cell lines after 24 hours. The ratios of apoptosis cell were significantly higher than that of controls (P<0.05) after curcumin influence the LNCaP and PC3 cell lines. Curcumin could arrest the cell cycle of LNCaP and PC3 cell lines at G2/M phase in does-dependent manners (P<0.01). The expression of IκBa in LNCaP cells had no significant difference after using curcumin (F=0.129 P>0.05). However, the expression of IκBa in PC3 cells increased gradually with the inducement of concentration-increased curcumin (F=31.618 P<0.01).Conclusions: Curcumine could suppress the growth of androgen-dependent and androgen-indenpent prostate cancer cell lines and promote their apoptosis by different mechanism.