Bcl-2 Small Interfering RNA Increased Sensitivity To 5-Fluorouracil In Gastric Adenocarcinoma Cells

Objective: To observe the expression of the Bcl-2 in gastric adenocarcinoma cells , to study its effection in the rate of proliferation. Changes in 5-fluorouracil (5-FU) sensitivity in Bcl-2 small interfering RNA (siRNA) transfected gastric adenocarcinoma cell line SGC-7901 were investigated in this study.Method: Set up control group, negative siRNA transfectants group, Bcl-2 siRNA transfectants group; Reverse transcription (RT)-PCR and western blot were used to detect the target gene expression at mRNA and protein levels, respectively. The proliferation inhibitory rate of gastric adenocarcinoma cell line SGC-7901 was assessed by MTT, and the apoptotic changes were examined by Propidium Iodide (PI) fluorescent staining and flow cytometry.Results: The Bcl-2 mRNA and protein expression levels in Bcl-2 siRNA transfectants were reduced compared with negative siRNA transfectants group or control group. MTT results showed that Bcl-2 siRNA transfected cells had a higher cell inhibition rate than negative transfected cells or control group after treatment with 15, 150, 1500, 15,000mg/L of 5-FU for 24h (P<0.05). Flow cytometry (FCM) results demonstrated that the Bcl-2 siRNA group had increased apoptosis rate compared with negative siRNA group and control group (P<0.05). After treated with 5-FU (1500mg/L), Bcl-2 siRNA transfected group showed a more increased apoptosis than negative siRNA group and control group (P<0.05) .Conclusions: siRNA targeting Bcl-2 gene can specifically down-regulate Bcl-2 expression in gastric adenocarcinoma cell line SGC-7901. siRNA targeting Bcl-2 gene by Bcl-2 specific siRNA could inhibit proliferation in gasgtric adenocarcinoma cell line SGC-7901 more effectively. siRNA targeting Bcl-2 gene can specifically down-regulate Bcl-2 expression in gastric adenocarcinoma cell line, and can increase spontaneous cell apoptosis and sensitize cells to 5-FU.