Bcl-2 Gene Silence Enhances The Sensitivity Toward 5-Fluorouracil In Gastric Adenocarcinoma Cells

Objective: To investigate the effect of Bcl-2-si RNA on the proliferation of gastric adenocarcinoma cell line SGC-7901; and to explore the feasibility to improve the therapeutic efficacy of chemoreagents such as 5-FU for gastric adenocarcinoma, with combinatory administration of Bcl-2 si RNA, as well as the possible mechanisms for the outcome.Method: Set up control group, negative si RNA transfectants group, Bcl-2 si RNA transfectants group; Reverse transcription(RT)-PCR and western blot were used to detect the target gene expression at m RNA and protein levels, respectively. The proliferation inhibitory rate of gastric adenocarcinoma cell line SGC-7901 was assessed by MTT, and the apoptotic changes were examined by Propidium Iodide(PI) fluorescent staining and flow cytometry.Results: The Bcl-2 mRNA and protein expression levels in Bcl-2 si RNA transfectants were reduced compared with negative si RNA transfectants group or control group. MTT results showed that Bcl-2 si RNA transfected cells had a higher cell inhibition rate than negative transfected cells or control group after treatment with 15, 150, 1500, 15,000mg/L of 5-FU for 24h(P<0.05). Flow cytometry(FCM) results demonstrated that the Bcl-2 si RNA group had increased apoptosis rate compared with negative si RNA group and control group(P<0.05). After treated with 5-FU(1500mg/L), Bcl-2 si RNA transfected group showed a more increased apoptosis than negative siRNA group and control group(P<0.05).Conclusions: si RNA targeting Bcl-2 gene can specifically down-regulate Bcl-2 expression in gastric adenocarcinoma cell line SGC-7901. si RNA targeting Bcl-2 gene by Bcl-2 specific si RNA could inhibit proliferation in gasgtric adenocarcinoma cell line SGC-7901 more effectively. si RNA targeting Bcl-2 gene can specifically down-regulate Bcl-2 expression in gastric adenocarcinoma cell line, and can increase spontaneous cell apoptosis and sensitize cells to 5-FU.