The Experimental Study Of The Effects And Mechanism Of Raphanus Sativus.L Extract On Gastrointestinal Motility

Objective:The study mainly utilize the raphanus which is a edible Chinese officinal herb and has little side-effect, easily accepted, steadly effect, and so on, to exploit its pharmacological value on gastrointestinal motility. By observing the effects of the crude extract of radish(Ecr)on the electrogastrogram(EGG),gastric emptying and small bowel peristalsis activity of the rats in vivo ,the effects of Ecr on gastrointestinal motility could be manifested. Furthermore, the contrast study about the effects of gastrointestinal motility was also observed among Ecr domperidone and prepulsid. The mechanism was explored by observing Ecr on the expression of Fos protein of the dorsal vagal complex(DVC).This study provided the expermental foundation and academic guidance to Ecr on the therapy of the functional disease on the gastrointestinal motility.Methods:Ecr was obtained by using rotary evaporation method . The rats of different groups were administered randomly with 0.3ml/kg,1 ml/kg,3 ml/kg of Ecr respectively. The change of the EGG ,the residue rates of pigment in stomach and propulsive proportion of small intestine of dextran blue-2000 in rats Were observed.The capacity of gastric emptying and small bowel peristalsis which were induced by Ecr could be calculated. Moreover, based on the optimal dosage got from pre-experiment, the contrast observation on the change of gastric emptying after being administered with Ecr and domperidone were carried out, and the change of the small bowel peristalsis of the rats after being administered with the Ecr and prepulsid were also observed. Finially, the mechanism that Ecr's promoting gastrointestinal motility was learned through observing the expression of Fos protein of the DVC by adopting the immunohistochemistry method.Results:â—‹1 Compared with the normal control group , Ecr could improve electrogastrogram(EGG)by enhancing amplitude at 0.3ml/kg,1 ml/kg,3 ml/kg, the difference is significant(P<0.01 ) .The most obviously change was the group of 1 ml/kg ,comparing to the other group , the difference was significan(tP<0.05).There existed no differecnce between the group of the 0.3ml/kg and 3 ml/kg(P>0.05);â—‹2 Ecr could increase gastric emptying at 0.3ml/kg,1 ml/kg,3 ml/kg, compared to the normal control group ,the difference is significant(P<0.01);the effect was most obvious at the group of 1 ml/kg ,comparing to the other group , the difference is also significan(tP<0.01);â—‹3 Ecr could enhance the small bowel peristalsis at 0.3ml/kg,1 ml/kg ,comparing to the normal control group ,the difference is significant(P<0.01).However, the group of the 3 ml/kg didn't manifest improvement role in the small bowel peristalsis, comparing to the normal control group, the difference is not significant ( P > 0.05 ) ;â—‹4 Compared with domperidone , the change of the gastric emptying induce by Ecr was no difference(P>0.05); the difference of the small bowel peristalsis of the rats by Ecr(P>0.05)is also no significant, compared with prepulsid.â—‹5 Ecr at the 1ml/kg could make the expression of the Fos protein of the DVC evidently change at some time, Compared to the control group ,the difference is significant(P<0.01).The expression of Fos protein was most significant at the group of 30min after administering with 1ml/kg,compared with other groups, the difference is also significant(P<0.01).Conclusion:Ecr could promote the gastrointestinal motility, which has some dose-effect relationship. The effect on the gastrointestinal motility of Ecr has no significant difference with the domperidone and prepulsid. The mechanism may be that Ecr could affect the activity of the DVC in the spinal cord, and sequentially the activity of pneumogastric nerve were regulated.