Four Gentlemen Decoction Extract And Its Mechanism Of Regulation Of Gastrointestinal Movement

Spleen-deficiency syndrome is a pathologic status that digestive and absorptive functions are decreased, enginery of gastrointestinal motility is turbulence, involved in many system's dysfunction, such as vegetative nerve, endocrine and immunization. At present, the disease of gastrointestinal dyskinesis has caused more and more scholar's attention and interesting. Many scholars have found that the deficiency of spleen is ralated to gastrointestinal dyskinesis. Si Jun Zi Decoction is a classic prescription that can treat the deficiency syndrome of the spleen. It comes from the third volume of ((Tai Ping Hui Mm He Ji Ju Fang)) . It comprises Ren Shen, Zhi Gan Cao, Fu Ling and Bai Zhu that they are divided in equal quantity. After the four drugs are made to power, six gram power are decocted with water and taken. Si Jun Zi Decoction's function is tonifying the Qi with the sweet and warm, strengthening the spleen and reinforcing the stomach. It can treat the deficiency of Ying Wei Qi, weak-willed Zang and Fu, fullness of the heart and abdomen, anorexia, borborygmus, diarrhea and vomiting. Over the years, pharmacological research in Si Jun Zi Decoction is a focal point in research with pharmacological about the prescription regulating the deficiency of the spleen. Si Jun Zi Decoction have dual action to rabbit ileum motivity in vitro, while the mechanism is not very definitude. In this essay , the effection of the different distillation of Si Jun Zi Decoction were screened on gastrointestinal motility. We especially do pharmacological research on gastrointestinal motility with normal butyl alcohol distillation of Si Jun Zi Decoction. We expect to explore the mechanism of Si Jun Zi Decoction to gastrointestinal motility.1.The different distillation of Si Jun ZI Decoction act on mouse diarrhea duo to senna:Using mouse diarrhea model with senna, we studied the effect of checking diarrhea with general distillation, acetic ether distillation, normalbutyl alcohol distillation, general polysaccharide, chloroform distillation of Si Jun Zi Decoction. Every reagent was diluted 10% solution. Result is that the effect of normal butyl alcohol distillation is the best and there is obviously dose-effect relationship.2.Research for action on gastrointestinal motility and its mechanism with the normal butyl alcohol distillation of Si Jun ZiDecoction:2.1 Methodological assessment on entogastric and enteral pressure determination model in vitroThis model was improved referencing rat gastric muscle strip in vitro and water sac perfusion, as well as vermiculation of guinea pig. The apparatus is the PC POLYGRAF HR(multipurpose data acquisition device, 8 pressure channels measurement). The experimental results show that acetylcholine could improve rat entogastric pressure and histamine could heighten enteral pressure of guinea pig in vitro. The both show evident dose-effect relationship (P<0.01-O.OO1) and (P