Study On Association Between LDL Receptor-related Protein 5 Gene Polymorphisms And Bone Mineral Density In Anhui Postmenopausal Women

Objective To investigate the association of Q89R and A1330V polymorphisms in low-density lipoprotein receptor-related protein 5 (LRP5) gene with bone mineral density (BMD) in Anhui postmenopausal women.Methods Q89R and A1330V genotypes were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 247 postmenopausal women in Anhui. BMD at lumbar spine 2-4 and the left proximal femur were measured by dual-energy X-ray absorptiometry in all subjects.Results The distribution of Q89R and A1330V genotypes in this population was as follows: QQ 83.4%, QR 16.6%, RR 0%; AA 70.9%, AV 26.3%, VV 2.8%. Q89R and A1330V polymorphisms were in linkage disequilibrium in the subjects(χ~2=24.48,P<0.01). BMD at the femoral neck, ward's and trochanter was significantly lower in subjects with Q89R QR genotype than in the combined group with QQ genotype. BMD at the femoral neck was significantly lower in subjects with A1330V AV/VV genotype than those with AA genotype. Q89R polymorphism was significantly associated with BMD at the femoral neck and trochanter(P<0.05) after adjusting for age, height, weight, BMI and years since menopause. No significant association was found between A1330V polymorphism and BMD at any site after adjusting these impact factors.Conclusion Q89R polymorphism in LRP5 gene may have an influence on BMD in postmenopausal women, which suggests that LRP5 gene is a candidate for the genetic determination of BMD.