| Background:Acute pancreatitis, especially severe acute pancreatitis, is an issue that can't besatisfied-solved in acute abdomen surgery. Recent researches suggest that apoptosis ofpancreatic cells may be one of the protection mechanisms in acute pancreatitis body, and it playsan important role in the progression of AP. Therefore, it is significant to identify the relationbetween apoptosis of pancreatic cells and progression of AP, to study the mechanism ofpancreatic cells' apoptosis and effective drug treatment in order to reduce the fatality of SAP inclinical treatment.Objective:To investigate the relation between the apoptosis of pancreatic cells and severity of SAP inrats, to observe the apoptosis index and the expression of Bax and Bcl-2 gene inResveratrol-treated SAP rats. The histopathological changes and wet/dry ratio of pancreas andserum amylase would also be detected. To explore the mechanism of apoptosis in SAPpancreatic cells and an effective way of drug treatment with SAP.Methods:Forty-eight Wistar rats were randomly divided into Resveratrol-treated group (n=20), SAPmodel group (n=20) and normal control group (n=8). Establishment of SAP model: 20% L-argsolution was first given to rats by intraperitoneal injection (450mg/100g body weight of rat) andsecond given by subcutaneous injection (100mg/100g body weight of rat) two hours later. RESgroup: Resveratrol solution was immediately given to rats by intraperitoneal injection (25mg/Kgbody weight of rat) after the SAP model was established. Rats of SAP group were immediatelygiven the DMSO solution by intraperitoneal injection after the establishment of SAP model.Rats of NS group were given the equal NS solution by the same way as establishment of SAPmodel. Five rats of SAP group and RES group were sacrificed respectively at 6,12,24,48 hoursafter the last injection while two rats in NS group. The changes of serum amylase and wet/dryratio of pancreas were detected. Pancreatic histopathological score based on microscopicchanges was evaluated. The expression of Bax and Bcl-2 gene protein in preacreas was observed by immunohistochemistry SP method. Apoptosis index of pancreatic cells was detected byterminal deoxynucleotidyl transferase-mediated dUTP-biotion nick end labeling (TUNEL)technique. The data were in MEAN±SD ((?)±s) and statistics analysis was made by using SPSS11.5 software for windows.Results:(1) The serum amylase of SAP group increased at 6 hour, peaked at 24 hour, and thendecreased. Compared RES group with SAP group, resveratrol can obviously decrease the serumamylase of SAP rats at 12 hour (P＜0.05) and at 24 hour (P＜0.01).(2) Compared with the NS group, RES group and SAP group have the significant statisticmeaning (P＜0.01). Resveratrol significantly decreased the wet/dry ratio of pancreas of rats withSAP at 24 and 48 hour (P＜0.05).(3) The histopathological changes of pancreas in RES group and SAP group rats appearedat 6 hour, peaked at 48 hour. Compared the RES group with the SAP group, thehistopathological changes of pancreas softly lessened but has no statistic meaning (P＞0.05) at 6and 12 hour while it has statistic meaning at 24 and 48 hour (P＜0.05).(4) The expression of Bax and Bcl-2 gene protein of preacreas is low in NS group whilehigh in RES group and SAP group. The expression is much higher in RES group than in SAPgroup, there is significant difference between them (P＜0.01) at 6 and 12 hour, but there is nodifference between them at 24 and 48 hour.(5) The apoptosis cells of pancreas increased enormously in SAP group and RES group.The apoptosis index which has great difference between RES group and SAP group at 6, 12, 24hour while no difference at 48 hour is much higher in RES group (6h, 12h, P＜0.01; 24h, P＜0.05;48h, P＞0.05).Conclusion:There is a negative correlation between apoptosis of pancreatic cells and severity in AP.Resveratrol can induce pancreatic cells apoptosis in SAP rats, reduce the histopathologicaldamage of pancreas and the serum amylase, decrease the pancreatic wet/dry ratio. Themechanism may be via up-regulation of the expression of Bax gene protein and Bcl-2 geneprotein. Resveratrol can effectively induce the apoptosis of pancreatic cells with SAP, it wouldprovide a possible way to reduce the severity and fatality of SAP in clinical treatment. |
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