The Effect Of Exogenous Progesterone Supplement On Immunology During Embryo Implantation

Objective: To study the effect of exogenous progesterone supplement on immunology during embryo implantation by observing the differences of the expression of progesterone-induced blocking factor> estrogen receptor-a and progesterone receptor in mice endometrium and serum estradiol and progesterone level after exogenous progesterone supplement.Methods: Kunming mice were divided into 3 groups, progesterone,dydrogesterone was respectively given to two groups from pregnant day 1 , the other group was controlled. Blood and Uterus samples of Kunming mice were collected on pregnant day 4,5,6. C57BL/6 mice were divided into 2 groups: syngeneic group ( C57BL/6×C57 BL/6 ) and allogeneic group(C57BL/6×BALB/c). Blood and Uterus Samples of C57BL/6 mice were collected on pregnant day 6. PIBF protein,estrogen receptor-a (ER-a)and progesterone receptor(PR) was measured with immuohistochemistry. Serum estradiol and progesterone level were detected by radioimmunoassay.Results: (1)serum level of E₂,P and the expression of PIBF,ER-a,PR in allogeneic pregnant mice was higher versus syngeneic pregnant mice(p<0.05). (2)Exogenous progesterone and dydrogesterone up-regulated the expression of PIBF (p<0.05), highly increased in dydrogesterone group (p<0.05). Serum P level increased significantly (p<0.01) but the expression of ER-a and PR decreased in progesterone group (p<0.05), however, Serum level of E₂ and P were not changed (p>0.05) but the expression of ER-a and PR increased in dydrogesterone group (p<0.05).Conclusion: the expression of PIBF requires allogeneic induced. Dydrogesterone up-regulated the expression of PIBF and the effect was stronger than progesterone. Dydrogesterone up-regulated but progesterone down-regulated the expression of ER-a and PR.