Exploration Of Hyperhomocysteinemia On Oxidative Stress And Inflammation Injury In Patients With Cerebral Infarction

Objectives: To study the correlations between plasma tHcy level and serum MDA,IL-8 levels,serum SOD vigor in patients with cerebral infraction, in order to explore the possible mechanism of Hyperhomocysteinemia on oxidative stress and inflammation injury in cerebral infraction.Methods: 119 hospital patents with acute cerebral infarction were chosen for our study, which were sicken in three days, in 2005-2006 years. They were all comfirmated by head CT or MRI, without atrial fibrillation,hepatophy,nephrosis,thyroid giand disease,infection and autoimmunity disease. 40 patents without cerebrovascular disease,hepatophy,nephrosis,thyroid giand disease,infection and autoimmunity disease were chosen for the control subjects, the age and sex of which were matched with the case group. According to history and manifestation of CT or MRI, the case group was divided into initial cerebral infarction group and recurrent cerebral infarction group, the recurrent cerebral infarction group was also divided into silent cerebral infarction with recurrent cerebral infarction group and symptomatic cerebral infarction with recurrent cerebral infarction group. On the basis of plasma tHey level, the case group was divided into Hhcy group and Nhhcy group. The plasma tHcy level was measured by high performance liquid chromatography methods, meanwhile serum SOD vigor,serum MDA,IL-8 levels were detected by xanthine oxidase,thiobarbituric acid reactive substance and double-antibody radio-immunity. Serum lipids and glucose were examed by TBA-120FR automatic analyse machine.Results: 1. Between the the case group and the control group, the initial cerebral infarction group and the recurrent cerebral infarction group,the Hhcy group and the Nhhcy group in the case group,the initial cerebral infarction group,the recurrent cerebral infarction group,the silent cerebral infarction with recurrent cerebral infarction group,the symptomatic cerebral infarction with recurrent cerebral infarction group, there had no statistical significance of smoking,drinking,diabetes mellitus,hypertension,coronary heart disease,Glu,TG,CHO(P＞0.05).2. There had statistical significance of Plasma tHcy level,serum MDA,IL-8 levels,serum SOD vigor in the case group and the control group(P＜0.01). Plasma tHcy level, serum MDA,IL-8 levels of the case group were respectively 19.97μmol/L,4.41±0.84nmol/Land 0.23±0.08ng/ml, which were significantly higher than those of the control subjects(9.83μmol/L,3.24±0.64 nmol/L,0.12±0.04ng/ml). Serum SOD vigor in the case group(87.05±12.86U/ml) was significantly lower than that of the control subjects(105.39±13.69U/ml).69.7％Hhcy patients were found in the case group, but only 5％Hhcy patients were found in the control subjects, the difference between them proved to be with statistical significance (P＜0.01).3. There had statistical significance of Plasma tHcy level,serum MDA,IL-8 levels,serum SOD vigor in the initial cerebral infarction group and the recurrent cerebral infarction group(P＜0.05). Plasma tHcy level,serum MDA,IL-8 levels of the initial cerebral infarction group(16.72μmol/L,4.19±0.58 nmol/L,0.21±0.06ng/ml) were lower than that of the recurrent cerebral infarction group(20.01μmol/L,4.52±0.77 nmol/L,0.24±0.08ng/ml), the former serum SOD vigor(90.53±12.86U/ml) was higher than the latter(85.23±12.56U/ml).4. In the case group,the initial cerebral infarction group,the recurrent cerebral infarction group,the silent cerebral infarction with recurrent cerebral infarction group and the symptomatic cerebral infarction with recurrent cerebral infarction group, the difference between the Hhcy group and the Nhhcy group about Plasma tHcy level,serum MDA,IL-8 levels,serum SOD vigor was proved to be with statistical significance(P＜0.05). Plasma tHcy level,serum MDA,IL-8 levels of the Hhcy group were higher than those of the Nhhey group, serum SOD vigor of the Hhcy group was lower than that of the Nhhcy group.5. Spearman correlation analysis results showed that plasma tHcy level was correlated with sex(p＜0.05), besides it was also correlated with serum MDA,IL-8 levels,serum SOD vigor(p＜0.01), but it was not correlated with age,smoking,drinking,diabetes mellitus,hypertension,coronary heart disease,Glu,TG,CHO(p＞0.05).Conclusion: Blood vessel oxidative stress and inflammation injury were proved to be aggravating with the increase of plasma tHcy level, Hyperhomocysteinemia cause cerebral infarction and recurrent cerebral infarction possibly by oxidative stress and inflammation injury.