The Expression Of P21 And Cox-2 In Human Astrocytoma

Glioma is the most frequent malignancy in central nervous system, which is one of the hot spots that were investigated by neurosurgeon. Astrocytoma is the most frequent in all type of gliomas.Many methods have been to attempt to treat glioma, now these methods are progressing, but we still have no way to change the state that the patients have high recurrence rate and high death rate.we have not cleared the mechanism how glioma formate and devel.With the progress of molecular biology and cellular genetics, especially on the level of gene, our understanding of malignancy had advanced dramatically in the past years.People come to know that it is an incident of multiple genes and multiple steps.Many Proto-oncogene and anti-oncogene construct a complicate network system, which accommodate corpuscular generation and differentiation. The control key points of each phase in cell cycle have important contribution. If any one of the component elements is abnormal, the malignancy would genesis possibly. P21 is the significant regulating factor in cell cycle, which expression would become a improtant parameter to estimate the prognosis of neurogliocytoma. The mutational RAS gene induce proto-oncogene activation, P21 change to activated to participate tumorous genesis and development. COX-2 is the rate-limiting enzyme in the process that arachidonic acid is composited to PG. COX-2 is important in the process. The overexpression of COX-2 can induce to PG level to step up,which is important in many types of tumorous genesis.Some factors induce the COX-2 expression to raise, then the VEGF expression would be up-regulation, which could stimulate tumorous angiogenesis.We detect the P21 and COX-2 expression in human astrocytoma with immunohistochemistry. Our topic intend to approach how the two affect the genesis and development of astrocytoma.Objective:To investigate the P21 and COX-2 expression in human astrocytoma and approach how the two affect the genesis and development of astrocytoma.Method:We manufacture human astrocytoma histological section of 64 examples, which include 14 examples of I grade,18 examples of II grade, 17 examples of III grade, and 15 examples of IV grade. We detect the P21 and COX-2 expression with immunohistochemistry. Another 6 examples of health adult brain tissue section get the same as up detection.Result1,Normal brain tissue have no P21 expression, and the label index of expression is higher in high grade astrocytoma than low grade astrocytoma.The difference of the two is significant. (P<0.05).2,Normal brain tissue have no COX-2 expression.The evidence suggests that COX-2 expression in the astrocytoma could be associated with the grade.High expression associated with high grade. The dye index of each grade have significant difference. (P<0.05)Conclusion1,The P21 expression is higher in both III grade and IV grade than in both I grade and II grade. The deviation is significant. P21 can be a index which help to judge the malignant degree and prognosis of astrocytic.2,COX-2 expression in the astrocytoma could be associated with the grade. High expression associated with high grade. We could judge the pathograde of astrocytoma by the dye index of COX-2.The generation of neoplastic cell and the growth of neoplastic vassular could be inhibited if we inhibit the expression of COX-2, and by the way we could delay the tumorous growth, recurrence and canceration.