The Effect Of "Shu XueTong" On VEGFR-2 And SRF Expression

1, Study objectivesTo determine whether the traditional Chinese drug "ShuXueTong" have effects on vascular endothelial growth factor receptor (VEGFR-2) and serum response factor (SRF) expression, and to explore what mechanism it through to promote the growth of vessel.2,Materials and Methods1,1 MaterialsImmortal human umbilical vein endothelial celllines, RPMI 1640 added 10%FBS, anti-VEGFR-2 IgG anti-SRF IgG, and traditional Chinese drug "ShuXueTong" injection.1, 2 methods1, 2, 1 cell cultureImmortal human umbilical vein endothelial cells were cultured withRPMI 1640 added 10%FBS in culture flasks. At the third passage, the endothelial cells were transfered to some coverslips and cultured in 6-well culture flasks at a density of 30000/ml.1,2, 2 groupsTreated groups: "ShuXueTong" injection(1:16 dilution) were dropped into the cutlture solution to intervene the growth of human umbilical vein endothelial cells.Control groups: Normal sodium were added into the culture solution.1, 2, 3 DetectionsThe expression of VEGFR-2 and SRF were respectively detected with immunocytochemistry stain after a certain time line of 24h, 1d, 2d, 3d, 4d, 5d, and 6d.1, 2, 4, ObervationsThe stained cells were observed under high power lens. We obvered five visual fields at random in each slip and counted the positive cells in every visual field. The general average of the positive cells in the five visual fields were taken as the result.1, 2, 5 statisticsThe data of the treated groups and control groups were analyzed with Student's t test.3, ResultsCompairing with the control cells, the expression of VEGFR-2 and SRF in treated cells was dramatically enhanced (P<0.001) .4, ConclusionsThese results suggest that "ShuXueTong" can enhance the expression of VEGFR-2 and SRF of VECs, and it may promote the growth of vessel by inducing VECs multiplication and vascular smooth muscle cell differentiation.