Study On The Chemical Constituents And Bioactivities Of Adina Rubella Hance.

The chemical constituents and biological activities of Adina rubella Hance were studied in this project.Four compounds were isolated from Adina rubella Hance with methods of column chromatography, thin layer chromatography and recrystallization. Two of the four compounds were identified as 5,7-dihydroxy-2-methylchromone and scopoletin respectively by IR, ¹HNMR, ¹³CNMR, LC-MS analysis. The chemical structures of the other two compounds are still being studied. Based on IR and GC-MS analysis, one mixture of alipatic acid was identified as: palmitic acid ethyl ester, ethyl-9,12-octadecadienoate, 9,12,15-octadecatrienoic acid ethyl ester, octadecatrienoic acid ethyl ester and 2,6,10,14,18,22-teracosahexaene; another mixture ofsterols has been identified as stigmastan-3,5-diene, stigmast-5-en-3-ol, stigmasta-3,5-diene-7-one and stigmastan-5,22-diene-3-ol.Antibacterial activity of alcohol extract of Adina rubella Hance and its solvent fractions against 6 bacteria strains was studied. Minimal inhibitory concentration (MIC) was used to determine the antibacterial effects of the samples. The results indicated that the sterol mixture and ethyl acetate fraction showed in vitro inhibition against a few bacteria strains in higher concentration.The antivirus of alcohol extract of Adina rubella Hance and its solvent fractions was studied with methods of CPE and MTT. The inhibitory effect on HSV-1, CoxB3 is not good, while the toxic effect is higher.